We used preS2-S'-beta-galactosidase, a three-domain fusion protein that aggregates extensively at 43 degrees C in the cytoplasm of Escherichia coli, to search for multicopy suppressors of protein aggregation and inclusion body formation and took advantage of the known differential solubility of preS2-S'-beta-galactosidase at 37 and 43 degrees C to develop a selection procedure for the gene prod...

in the present study, we investigated total cell protein patterns of ten isolates of epidermophyton floccosum var.pigmented by sds-page on 10% polyacrylamide resolving gels. densitometric analysis of the gels allowed to detect more than 31 clearly detectable mycelial protein bands with molecular weights in the range of 12 to 98 kd proteins of 12.5, 13.5, 14.4, 16, 18.4, 19.7, 20.1, 23.5, 26, 27...

Two peptic fragments (residues 37-88 and 43-88) of guinea pig myelin basic protein which are capable of inducing experimental allergic encephalomyelitis in Lewis rats were cleaved to shorter fragments with alpha-protease (Crotalus atrox proteinase, EC 3.4.24.1) and thermolysin (EC 3.4.24.4). The fragments were isolated, purified, and identified by amino acid composition and NH2- and COOH-termin...

We have examined the ability of misonidazole (MISO) or etanidazole (ETA) to improve the antitumor efficacy of cisplatin (CDDP), hyperthermia, and radiation in the FSaIIC murine fibrosarcoma. A growth delay of about 25 days was produced with CDDP (5 mg/kg) and hyperthermia (43 degrees C, 30 min) prior to radiation (3 Gy daily for 5 days) on day 1. The addition of MISO (1 g/kg) on day 1 resulted ...

Twenty-four multiparous lactating Holstein cows were blocked by days in milk and assigned to treatment sequences in a replicated 4x4 Latin square with 21-d periods. The four diets, formulated from alfalfa silage plus a concentrate mix based on ground high moisture ear corn, contained [dry matter (DM) basis]: 1) 20% concentrate, 80% alfalfa silage (24% nonfiber carbohydrates; NFC), 2) 35% concen...

3 Semilinear equations with absorption 19 3.1 The Marcinkiewicz spaces approach . . . . . . . . . . . . . . . . . . . . . . 21 3.2 Admissible measures and the ∆2-condition . . . . . . . . . . . . . . . . . . . 26 3.3 The duality method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 3.3.1 Bessel capacities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 3.3.2 Sharp...

We investigated the effect of temperature (19, 30, 37, and 43 degrees C) on the p50 value for normal human blood at pco2 = 5.72 kPa (43 mmHg), at various pHs (range 7.0 to 7.6) and molar ratios of [2,3-diphosphoglycerate]/[Hb4] (range 0.4 to 2.4). The d(log p50)/d(pH) coefficient varied from 0.39 at 19 degrees C to 0.35 at 43 degrees C. The relationship between log p50 and 1/T (T = degrees Kelv...

3 Semilinear equations with absorption 19 3.1 The Marcinkiewicz spaces approach . . . . . . . . . . . . . . . . . . . . . . 20 3.2 Admissible measures and the ∆2-condition . . . . . . . . . . . . . . . . . . . 26 3.3 The duality method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 3.3.1 Bessel capacities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 3.3.2 Sharp...

.............................................................................................................. 3 Introduction ......................................................................................................... 5 Chapter 1: Züllinger und seine Zucht and The New Race of Devils: Eugenics and artificial reproduction in the dystopias of the early twenties .........................

The effect of various sulfhydryl compounds on the survival of exponentially growing monolayer cultures of Chinese hamster cells (HA1) heated to temperatures of 37-43 degrees was examined. Concentrations of cysteamine which were nontoxic or minimally toxic at room temperature or 37 degrees became increasingly toxic at elevated temperatures, greatly potentiating the killing produced by heat alone...