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Article info: Received: 7 September 2015 Accepted: 19 October 2015 ABSTRACT Proteases are ubiquitous in occurrence and are found in all living organisms. These are essential for cell growth and differentiation. The extracellular proteases are of a high commercial value and find multiple applications in various industrial sectors. The present study describes the screening of protease producing b...
A peripherally inserted central catheter line was inserted and the patient received the first part of induction chemotherapy according to the GMALL 06/1999 protocol. The treatment course was uneventful and bone marrow aspirate at the end of the course was compatible with complete remission. On day 3 after the initiation of the second part of the induction course, the patient became febrile (38....
Considerable amount of work has been devoted to the identification and quantification of contaminants in paper and paperboard for food packaging applications, particularly in papers containing recycled fibre. However, much less effort has been dedicated to the development of predictive migration models for such materials when used in contact with food. The work presented here has as main object...
Neural networks and hybrid models were used to study substrate and product inhibition observed in the enzymatic hydrolysis of cellobiose at 40oC, 50oC and 55oC, pH 4.8, using cellobiose solutions with or without the addition of exogenous glucose. Firstly, the initial velocity method and nonlinear fitting with Statistica were used to determine the kinetic parameters for either the uncompetitive ...
Cratoxylum formosum Dyer is consumed throughout the year as food and medicine in Thailand. It contains large amounts of chlorogenic acid and quinic acid derivatives. The antioxidative activity of the extract was studied in refined soybean oil coating on rice crackers without any seasoning. They were stored in accelerated oxidation conditions at 40oC, 80% relative humidity (RH) in the dark for 1...
Ochratoxin A (OTA) is a naturally occurring fungal metabolite produced by some species of Aspergillus and Penicillium. OTA being nephrotoxic, carcinogenic, hepatotoxic and immunosuppressive imparts many human and animal health hazards. In this study OTA production was observed at different temperatures (5oC, 10oC, 20oC, 30oC and 40oC) in three substrates (corn, rice and wheat) by A. ochraceus a...
Xylanase production from Bacillus pumilus VLK-1 in solid state fermentation was enhanced through optimization of the process using one variable approach. The enzyme production was highest (42324±786 IU/g) upon 96h of incubation at 37°C using wheat bran as a substrate moistened with mineral salt solution (pH 9.0) containing 0.1% folic acid, 0.2% riboflavin and 6.0% yeast extract in the ratio of ...
Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out by means of solid state fermentation using orange bagasse, corn tegument, wheat bran and mango and banana peels as carbon sources. The maximal activity value of polygalacturonase (Pg) (30U.g) was obtained using wheat bran as carbon source while maximal pectin lyase (Pl) (2000 U.g)...
Molecular cloning and characterization of a bile salt hydrolase from Lactobacillus acidophilus PF01.
Phenotypic screening for bile salt hydrolase (BSH) activity was performed on Lactobacillus acidophilus PF01 isolated from piglet feces. A gene encoding BSH was identified and cloned from the genomic library of L. acidophilus PF01. The bsh gene and surrounding regions were characterized by nucleotide sequence analysis and were found to contain a single open reading frame (ORF) of 951 nucleotides...
The objective of the work was to study effect drying temperature on cup quality robusta coffee subjected mechanical in comparison with conventional sun drying. processed by wet (parchment coffee) and dry (cherry methods were at different regimes (40 oC, 50 oC 60 oC) a rotary dryer. results indicated that as increased, time reduced. Sun parchment took 48 hours (approximately seven days) attain d...
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