The aim of this study was to define the relation between silver stained nucleolar organizer regions (AgNORs) count and histological grade, and clinical stage in needle biopsy specimens of 65 prostatic adenocarcinomas. Histological grade was determined according to the modified Gleason system. AgNORs were evaluated by the counting method on routine histological sections stained with silver. Ther...

A cytogenetic study was conducted on five species of the genus Pimelodus collected from various regions in Brazil: Pimelodus heraldoi, Pimelodus sp, Pimelodus argenteus, P. maculatus and P. mysteriosus. All the species had 2n=56 chromosomes. AgNOR was found in one pair of subtelocentric (ST) chromosomes with staining at the terminal position in all the five species. However, Pimelodus sp, P. he...

AIM To determine the potential value of interphase cytogenetic and argyrophilic nucleolar organiser region (AgNOR) analyses in the diagnosis and classification of hydatidiform moles. METHODS Serial tissue sections from 37 hydatidiform moles, histologically classified as 11 complete and 15 partial, and from 11 hydropic abortuses were examined by in situ hybridisation using digoxigenin labelled...

AIM To investigate the prognostic value of argyrophylic nucleolar organiser regions (AgNORs) in multiple myeloma. METHODS Bone marrow aspirates from 55 newly diagnosed patients with multiple myeloma were stained with the one step AgNO3 technique. The mean number of AgNORs in each plasma cell nucleus (AgNOR count) was tested for a possible correlation with other clinical and laboratory variabl...

STUDY OBJECTIVES To evaluate the usefulness of argyrophilic nucleolar organizer region (AgNOR) counting and flow cytometric DNA analysis in the differential diagnosis of thymoma and thymic carcinoma, as well as in the differences among various stages and histologic subtypes of these tumors. DESIGN AND INTERVENTIONS Paraffin-embedded blocks of 64 thymic epithelial tumors (20 noninvasive thymom...

BACKGROUND Prediction of radiation response before the completion of the radiotherapy schedule is challenging. Information about radiation response could help oncologist to choose the appropriate combination and sequence of therapies in the multidisciplinary management of cancer. METHODS The study involved 26 patients with squamous cell cancers of the head and neck region who received radioth...

The study sought a correlation between the number of AgNOR granules and the degree of cervical intraepithelial neoplasia (CIN). Thirty-five sections (5 normal, 10 CIN1, 10 CIN2 and 10 CIN3) were subjected to retrospective analysis. The percentage of cells with 1, 2, 3, 4 and more AgNORs was calculated and the number of granules per 100 cells was counted. The number of cells containing single gr...

discrimination of regenerative cirrhotic nodules of the liver (cn) from hepatocellular carcinoma (hcc) is sometimes difficult. we examined the utility of agnor staining in this context. fifteen cases of hcc and 25 cases of cn were stained by agnor method and the mean agnor number for one hundred nuclei in each case was determined. there was a significant difference between the mean agnor counts...

BACKGROUND Proliferative potential of testicular tumor was assessed by immunohistochemistry using anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody and silver staining of argyrophilic nucleolar organizer region (AgNOR), and the results were compared with clinical course. METHODS Fourty-five patients with testicular tumor and 10 normal testicles were investigated. All specimen...

PURPOSE To evaluate the mutual inter-relationship of mitotic indices, argyrophilic nuclear organizer regions (AgNOR) count, Ki-67 and B-cell lymphoma 2 protein (bcl-2) in papillary urothelial bladder cancer (pUBC), and their correlation with grade and stage. To establish the cut-off values of these markers to detect high grade and muscle invasive bladder cancer. MATERIALS AND METHODS Fifty-fo...