mycobacterium tuberculosis (h37rv strain) was used in this study. the bacterial cells were disintegrated by sonication. the separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using sds and 2me. eight protein molecules with molecular weights ranging from 6.3 ...

Background: Brucellosis is one of the important zoonotic diseases with variety of symptoms and signs. Using of cost effective, easy and safe diagnostic methods in screening of brucellosis is an important priority. Several studies have been carried out to recognize appropriate diagnostic methods and each have reported different results. Aim: The aim of this study was to investigate the sensitivi...

We consider the problem of randomly distributed positive delta-function scatterers in a strong magnetic field and study the behavior of density of states close to the spectral boundary at E = h̄ωc/2 in both two and three dimensions. Starting from dimensionally reduced expression of Brezin et al. and using the semiclassical approximation we show that the density of states in the Lifshitz tail at ...

8 0h 3c = 1.097× 10 m−1 sets the scale of energy levels, and hence electonic transitions, in hydrogen. Outer electrons in more complex atoms often have energies typical of higher levels in hydrogen. • The fine structure constant α = e2 4π 0~c = 137.04 −1 characterises the strength of the electromagnetic interaction. In hydrogen, it sets the scale of perturbations to the non-relativistic Schrödi...

Reactions of 9,10-phenanthrenequinone (PQ) in toluene with [M(II)(PPh3)3X2] at 298 K afford green complexes, trans-[M(PQ)(PPh3)2X2] (M = Ru, X = Cl, 1; M = Os, X = Br, 2) in moderate yields. Reaction of anhydrous RhCl3 with PQ and PPh3 in boiling ethanol affords the dark brown paramagnetic complex, cis-[Rh(PQ)(PPh3)2Cl2] (3) in good yields. Diffusion of iodine solution in n-hexane to the trans-...

We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneous precipitation and denaturation with TCA and 2ME in cold acetone. We also describe protein solubilization prior to IEF, either in classical rod gels or in IPGs, using two different solutions. The procedure is easy to carry out. The major caveats are...