Examples of both anti-M and anti-N have been reported in patients possessing these antigens; however, in only two instances has anti-M been reported as an autoantibody. This paper describes two additional patients with auto anti-M. In each case the anti-M was shown to be an autoantibody by absorption and elution studies. Optimum reactivity was obtained at 4 C. Column chromatogaphy and 2-mercapt...

The characteristics of the intact factor VIII/von Willebrand factor protein binding to human platelets was compared to 2-mercaptoethanol-treated factor VIII/von Willebrand factor protein and to fractions of plasma factor VIII/von Willebrand factor protein that elute after the void volume. These studies indicate that the factor VIII/von Willebrand factor protein larger size oligomers bind prefer...

Apolipoprotein A-I exhibits a polymorphism that can be easily investigated in native serum by a simple method involving incubation of serum in the presence of decylsulfate and beta-mercaptoethanol and subsequent isoelectric focusing. From six to eight proteins can be separated in a pH gradient from 4 to 6 and thus patients with apolipoprotein A-I variants can be distinguished from normal person...

The photo-oxidation of lysozyme (hen’s egg white), sensitized by methylene blue in 84% acetic acid solution, causes the selective modification of the methionyl residues, which are converted to the sulfoxide. The photo-oxidized protein shows a drastically reduced enzymic activity. The data obtained by comparing the sensitivity to proteolytic enzymes, as. well as the accessibility of disulfide bo...

Antibody capable of sensitizing rabbit skin for passive cutaneous anaphylaxis is produced in the rabbit as early as 6 to 7 days following antigenic stimulation. It reaches peak activity around the 9th day and is gone by the 3rd wk. The antibody is heat labile, sensitive to treatment with mercaptoethanol, non-precipitating and does not fix complement. In order to demonstrate PCA activity a laten...

Sera from 173 turkeys, 108 chickens, and 48 ducks from Giza, Egypt, were tested for the presence of anti-Toxoplasma gondii antibodies by means of the modified agglutination test using mercaptoethanol and formalin-fixed tachyzoites. The prevalence of anti-T. gondii antibodies (>1:25) among turkeys, chickens, and ducks was 59.5%, 47.2%, and 50%, respectively.

immunofixation (2), and beta-mercaptoethanol reduction (3) of serum, with subsequent zone electrophoresis (Figure 1). In each instance, such reduction caused the band to disappear. The immunofixation and immunoelectrophresis procedures confirmed the presence of polymeric, polyclonal 1gM. One would expect that a discrete band would still be present on zone electrophoresis after reduction if 1gM ...

Human serum proteins were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis after protein denaturation in the presence or absence of 2-mercaptoethanol. Both electrophoretic techniques give characteristic and reproducible banding patterns and achieve a high degree of resolution within the limits of a one-dimensional separation. The major protein bands have been identified, a...