improvements on embryo micromanipulation techniques led to the use of embryo biopsy in commercial embryo transfer programs for genetic analysis of preimplantation bovine embryos. the aim of this study was to evaluate the quality of bovine blastocyst derived from embryos biopsied at different precompacted morulae stages by assessment of cryosurvivability of the resulting blastocysts. the in vitr...

background: this study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (tcm199)–vero cells and menezo b2 (b2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. materials and methods: bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. their presump...

The Fas-Fas ligand (L) system is one of the major signalling pathways to induce apoptosis in various cells and tissues. The aim of this study was to investigate the expression of the Fas-Fas L system in rat and human oocytes and preimplantation embryos. We determined the expression of Fas and Fas L mRNA of rat oocytes and embryos up to the blastocyst stage, and of human embryos at the 2- or 4-c...

Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...

background: morphine consumption during pregnancy could lead to defect and delay in nervous system development in the embryos. in the present study, development of the tongue of embryos whom their mothers received oral morphine during pregnancy have been studied. methods: female wistar rats (200-220 g) after pregnancy were divided randomly into the experimental and control groups. the control g...

abstract: introduction & objective: the infertility treatment by using in vitro matured oocyte has many potential applications. it minimizes the risk of ovarian hyper stimulation and is an alternative treatment for women with polycystic ovarian syndrome who may have problems regarding stimulation for ivf. this method may prove important for subjects in need of fertility preservation and also pr...

Background: Chromosome abnormalities are associated with poor morphology and development in human preimplantation embryos, all together lead to poor outcomes. This study aimed to explore altered expression of DNA damage pathways in “poor morphological and development embryos with sever aneuploidies”. Materials and Methods: Surplus day-4 embryos of PGD cases were pooled in two groups: Poor progn...

Introduction: In the present study, the effects of oral morphine consumption in pregnant female rats on the amygdaloid complex development in the embryos were investigated. Methods: Female Wistar rats weighing 250-300 g (n=15) were divided into control (n = 8) and experimental groups (n = 7). The experimental group received morphine (0.05 mg/ml) in their tap water. On the 19th day of pregnan...