The oxygen uptake which is observed when Rhodospirillum rubrum chromatophores are illuminated under air and in the presence of reduced 2, 6-dichlorophenolindophenol (DCIP), 2, 3, 5, 6-tetra-methyl-P-phenylenediamine (diaminodurene, DAD) or N, N'-tetramethyl-p-phenylenediamine (TMDP) depends on the electron-donor concentration according to the equation of Michaelis-Menten. The apparent Km for th...

D IAPHORASE is a flavin-containing enzyme which catalyzes the oxidation of DPNH (reduced diphosphopyridine nucleotide) in the presence of an electron acceptor. The procedure generally used for determination of the activity of this enzyme is based on spectrophotometric measurement of the reduction of 2,6-dichlorophenolindophenol at 600 mit. As pointed out by Mahler (4), this technic is not appli...

A new kinetic method for the determination of serum adenosine deaminase (EC 3.5.4.4) is described, with adenosine as the substrate and nucleoside phosphorylase and xanthine oxidase as the reaction enzymes. Inosine is produced, which is converted to hypoxanthine. The hypoxanthine is oxidized to xanthine, which is further oxidized to uric acid. In these two reactions, blue 2,6-dichlorophenolindop...

straightforward conversion of (+)-6,8-dimethoxy-3,4-dihydro-3- undecylisocoumarin (3) to the title isocoumarin was carried out. hydrolytic ring opening of (3) afforded the hydroxy acid (4) which was immediately oxidized to keto acid (5) using chromic acid, cyclodehydration of (5) afforded the 6,8- dimethoxy-3-undecylisocoumarin (6) which on selective demethylation of 8- methoxy group furnished ...

This overview article for the special series, "Bayesian Networks in Environmental and Resource Management," reviews 7 case study articles with the aim to compare Bayesian network (BN) applications to different environmental and resource management problems from around the world. The article discusses advances in the last decade in the use of BNs as applied to environmental and resource manageme...

New 5-hydroxy- and 5-chloro-6-alkyl-1,4-benzoquinones with one or two methyl groups on the nucleus were synthesized as potential antimetabolites of plastoquinones for biological research on photosynthetic and mammalian enzyme systems; the primary emphasis was on photosynthesis.2,3-Dimethyl-5-hydroxy-6-phytyl-1,4-benzoquinone completely inhibited in chloroplasts the water-dependent electron tran...

The application of an electrochemical sensor, based on a glassy carbon electrode, modified with a cellulose acetate polymeric film bearing 2,6-dichlorophenolindophenol (CA/DCPI-CME), for flow injection analysis of sulphide, is described. The overall reaction was found to obey a catalytic regeneration mechanism (EC mechanism) and the electrochemical rate constant k(f) for the electrocatalytic ox...