Inhibition of lycopene cyclization decreased the salt tolerance of the euhalophyte Salicornia europaea L. We isolated a β-lycopene cyclase gene SeLCY from S. europaea and transformed it into Arabidopsis with stable expression. Transgenic Arabidopsis on post-germination exhibited enhanced tolerance to oxidative and salt stress. After 8 and 21 d recovery from 200 mM NaCl treatment, transgenic lin...

Reagents. Oligonucleotides were synthesized and purified by Sangon Inc. (Shanghai, China). The sequences of these oligonucleotides are listed in Table S1. E.coli exonuclease III (Exo III) was purchased from Fermentas Inc. (Vilnius, Lithuania). Silver nitrates (AgNO3) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Sodium borohydride (NaBH4) was obtained from Tianlian...

Preparation and crystallization G6-amylase from alkalophilic Bacillus sp.707 was expressed by Bacillus subtilis 207-25. The enzyme was crystallized by the hanging drop vapor diffusion method using the reservoir solution containing 50% (v/v) 2methylpentane-2, 4-diol, 100 mM Tris-HCl (pH 8.5) and 200 mM ammonium phosphate. Calcium chloride and sodium chloride were added to final 1 mM in the drop ...