Simple sequence repeats (SSRs) are highly informative, polymorphic, and co-dominant Mendelian markers that provide an important genomic resource for genetic research. Recently, the use of large-scale transcriptome sequence has become a reliable and efficient approach for the identification and development of new EST-SSR markers. In this study, 8389 potential SSRs with a minimum of five repetiti...

White clover (Trifolium repens L.) is an allotetraploid species (2n = 4X = 32) that is widely distributed in temperate regions and cultivated as a forage legume. In this study, we developed expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers, constructed linkage maps, and performed comparative mapping with other legume species. A total of 7982 ESTs that could be assembled ...

Cross-species transferability and expressed sequence tags (ESTs) in public databases are cost-effective means for developing simple sequence repeats (SSRs) for less-studied species like medicinal plants. In this study, 11 EST-SSR markers developed from 742 available ESTs of Withania Somnifera EST sequences and 95 SSR primer pairs derived from other solanaceous crops (tomato, eggplant, chili, an...

PREMISE OF THE STUDY Transcriptomes were used to develop microsatellite markers for the plant genus Orinus (Poaceae), which comprises three species of grasses (O. thoroldii, O. kokonoricus, and O. intermedius) that are widely distributed in the Qinghai-Tibetan Plateau. METHODS AND RESULTS Primer pairs were developed for 16 high-quality simple sequence repeats (SSRs) using transcriptomes. SSRs...

Transfer of molecular markers is widely used in conservation genetic studies. We investigated the transferability of simple sequence repeat (SSR) markers developed for Enterolobium cyclocarpum to E. contortisiliquum, a tropical tree widely distributed in dry forests. A set of 9 evaluated SSR markers were amplified in E. contortisiliquum and the degree of polymorphism was assessed in 8 tre...

the genetic structure of the genus alburnus is not well known and the phylogenetic relationships among its species are uncertain. in the present study, simple sequence repeats (ssrs or microsatellites) were used to evaluate genetic diversity and genetic differentiation between alburnus mossulensis heckel, 1843 from kashgan river in lorestan province and alburnus caeruleus heckel, 1843 from gama...

Vigna reflexo-pilosa, which includes a neglected crop, is the only one tetraploid species in genus Vigna. The ancestral species that make up this allotetraploid species have not conclusively been identified, although previous studies suggested that a donor genome of V. reflexo-pilosa is V. trinervia. In this study, 1,429 azuki bean EST-SSR markers were developed of which 38 EST-SSR primer pairs...

The genetic structure of the genus Alburnus is not well known and the phylogenetic relationships among its species are uncertain. In the present study, simple sequence repeats (SSRs or microsatellites) were used to evaluate genetic diversity and genetic differentiation between Alburnus mossulensis Heckel, 1843 from Kashgan River in Lorestan province and Alburnus caeruleus Heckel, 1843 from Gama...

in order to study the genetic diversity among 35 accessions of ae. triuncialis l. from17 ssr loci of 56 primer pairs originated from a and d genomes of bread wheat were used. seventy one fragments amplified totally and 68 of them were polymorph between genotypes. allele number ranged from 1 to 8 with average 4.18 per locus. pic value of markers was from 0.593 (xgwm-30-2d, xgwm-383-3d, xgwm-654-...

A database of 30,137 EST sequences from Mycosphaerella graminicola, the septoria tritici blotch fungus of wheat, was scanned with a custom software pipeline for di- and trinucleotide units repeated tandemly six or more times. The bioinformatics analysis identified 109 putative SSR loci, and for 99 of them, flanking primers were developed successfully and tested for amplification and polymorphis...