The aim of this study was to explore the characteristics blaCTX-M-27 carriage and mobilization in Salmonella Escherichia coli isolates from food-producing animals China. A total 2280 E. 229 collected food June 2003 September 2014 were screened for presence gene. blaCTX-M-27-positive typed plasmid DNA sequenced determine genetic context types present. Bacterial fitness evaluated by growth curve ...

We screened 30 Klebsiella pneumoniae isolates from dogs and cats at a single animal hospital in Guangdong Province, China. Among them, 12 K. pneumoniae strains possessed high-level resistance to amikacin and gentamicin and these were screened for 16S rRNA methyltransferase (16S-RMTase) genes. And then the genes positive isolates were detected for ESBLs (extended spectrum β-lactamases) and analy...

BACKGROUND Plasmid encoded blaCTX-M enzymes represent an important sub-group of class A beta-lactamases causing the ESBL phenotype which is increasingly found in Enterobacteriaceae including Klebsiella spp. Molecular typing of clinical ESBL-isolates has become more and more important for prevention of the dissemination of ESBL-producers among nosocomial environment. METHODS Multiple displacem...

results of the 100 klebsiella isolates, 41 (41%) demonstrated resistance or reduced susceptibility to ceftazidime and/or aztreonam and 35% (n = 35) were esbl-producers. twenty-eight (8o%) of the esbl-producing isolates carried the blactx-m type genes. based on pcr assays and sequencing of blactx-m genes, ctx-m-1, ctx-m-2 and ctx-m-9 were identified in 21 (60%), 15 (42%) and nine (34%) of these ...

AIM To determine the prevalence and genotypic characterisation of extended spectrum beta-lactamases produced by gram negative bacilli isolated at Mbarara Regional Referral Hospital (MRRH). SAMPLES Gram negative clinical isolates. STUDY DESIGN Laboratory-based descriptive cross-sectional study. PLACE AND DURATION OF THE STUDY MRRH, June and August 2012. METHODS Gram negative clinical iso...

The Escherichia coli sequence type (ST) 131 C2/H30Rx clade with the blaCTX-M-15 gene had been most responsible for the global dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. ST131 C1/H30R with blaCTX-M-27 emerged among ESBL-producing E. coli in Japan during the late 2000s. To investigate the possible expansion of a single clade, we performed whole-genome sequencing for ...

AGGAATCCCATG-30 and CTX-M-R2, 50-GCCGTCTAAGGCGATAAAC-30, were used to amplify the entire allele (876 bp) for sequencing. This revealed that S. enterica serovar Senftenberg strain 9-4 produced CTX-M-3, which was first identified in Poland. Southern hybridization using the whole blaCTX-M gene as a probe showed that the gene was located on a plasmid of < 38 kb in size. Attempts to transfer this pl...

BACKGROUND The already high and increasing occurrence of extended-spectrum beta-lactamases (ESBL) producing Escherichia coli in European broiler populations is of concern due to the fact that third and fourth generation cephalosporins are deemed critically important in human medicine. In Sweden 34% of the broilers carry ESBL/pAmpC producing E. coli in their gut, despite the absence of a known s...

Background: Atypical enteropathogenic Escherichia coli (aEPEC) is regarded as a globally emerging enteropathogen. aEPECs exhibit various level of resistance to a range of antibiotics, which is increasing alarmingly. The present study investigated the antimicrobial resistance of aEPEC isolates recovered from diarrheal patients, healthy carriers, animals, and raw meats. Results: Among 267 aEPEC i...