Ten ACA yeast small nucleolar RNAs (snoRNAs) were shown to be required for site-specific synthesis of pseudouridine psi in ribosomal RNA. A common secondary folding motif for the snoRNAs and rRNA target segments predicts that site selection involves: (1) base pairing of the snoRNA with complementary rRNA elements flanking the site of modification, and (2) identification of a uridine located at ...

Archaeal box C/D sRNAs guide the 2'-O-methylation of target nucleotides using both terminal box C/D and internal C'/D' RNP complexes. In vitro assembly of a catalytically active Methanocaldococcus jannaschii sR8 box C/D RNP provides a model complex to determine those structural features of the guide:target RNA duplex important for sRNA-guided nucleotide methylation. Watson-Crick pairing of guid...

We revisit the following problem (along with its higher dimensional variant): Given a set S of n points inside an axis-parallel rectangle U in the plane, find a maximum-area axis-parallel sub-rectangle that is contained in U but contains no points of S. (I) We present an algorithm that finds a large empty box amidst n points in [0, 1]: a box whose volume is at least log d 4(n+log d) can be comp...

Two core small nucleolar RNP (snoRNP) proteins, Nop1p (fibrillarin in vertebrates) and Nop58p (also known as Nop5p) have previously been reported to be specifically associated with the box C+D class of small nucleolar RNAs (snoRNAs). Here we report that Nop56p, a protein related in sequence to Nop58p, is a bona fide box C+D snoRNP component; all tested box C+D snoRNAs were coprecipitated with p...

background: stroke is a leading cause of death all around the world, and ischemic stroke is considered to be the most common stroke type. toll-like receptors (tlrs) are important molecules for detection of both pathogen invasion and tissue damage. in this regard, the purpose of this study was to assess the expression level of tlr2 on monocytes in patients with ischemic stroke and to evaluate th...

Given a black box for f, a smooth real scalar function of d real variables, one wants to estimate [symbol: see text]f at a given point with n bits of precision. On a classical computer this requires a minimum of d + 1 black box queries, whereas on a quantum computer it requires only one query regardless of d. The number of bits of precision to which f must be evaluated matches the classical req...

In this paper we consider the following covering problem. Given a set S of n points in d-dimensional space, d 2, nd two axis-parallel boxes that together cover the set S such that the measure of the largest box is minimized, where the measure is a monotone function of the box. We present a simple algorithm for nding boxes in O(n log n + n d?1) time and O(n) space.

S U M M A R Y Two-dimensional (2-D) finite-difference (FD) synthetics, which fill the gap between fast 1-D analytic synthetics and time-consuming full 3-D synthetics in our ability to model seismograms, have been used in many studies. We address several issues involving 2-D FD methods in generating global synthetic seismograms. These include: (1) interfacing point source excitation for earthqua...