Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands...

background zoonotic cutaneous leishmaniasis (zcl) is polymorphic disease that may show various clinical manifestations. objectives this study investigates the determination of genetic variation within the species of leishmania major isolates from new cases in chabahar, a port city in southeast iran (situated at the iran-pakistan border). migration in this region indicates that leishmaniasis is ...

in this study 19 blv infected and 2 healthy lymph nodes were analysed by western blotting to detect viral p 24 antigen . western blotting was carried out by rabbit anti p24 serum and p24 monoclonal antibody. rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kd bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kd bands. our results indic...

Due to lack of morphological methods to identify sex at early stage in the plants with long juvenile period the application of molecular markers is expected to facilitate breeding program. The objective of this study is to identify molecular markers linked to sex determination of the plant Simarouba glauca which assists in crop improvement program. Random amplified polymorphic DNA primers were ...

the genetic diversity of 40 tobacco (nicotiana tabacum l.) cultivars was analyzed using 12 issr primers. thecultivars were evaluated for 14 traits using a simple lattice design (7×7) with two replications. of 149 amplified bands, 142 (95.3%) were polymorphic. maximum and minimum bands were generated by primers ubc825 (17 bands and 94.11% polymorphic) and ubc824 (7 bands and 100% polymorphic), r...

In the present investigation 28 genotypes of soybean were analysed using 37 start codon targeted (SCoT) markers and primers produced 260 DNA fragments with an average 7.03 bands per primer. From these primers, SCoT 33 65 wos most polymorphic, where 10 polymorphic amplification products detected. The lowest number amplified (2) was detected by 8, 14, 19, 31 59. Of bands, 200 (77.27 %) 5.41 To de...

saffron (crocus sativus) is the most valuable and indigenous crop in iran. the stigmas of flower are used as a popular natural flavouring, colouring and medicinal agent. however, the market suffers from frauds in this plant such as mixing with safflower petals due to high profit. identification of these frauds with conventional and biochemical methods is difficult and low sensitive. therefore, ...