In November 2017, a group of farmed rainbow trout (Oncorhynchus mykiss) died in Yunnan Province, China, likely due to infection. These exhibited slow movement, no feeding, dark body color, exophthalmia, and occasional ulcers on the surface. Pathogens were isolated from diseased livers, head kidneys, spleens, eyes, strain was preliminarily identified as Acinetobacter johnsonii based morphology, ...

The outer membrane protein (Omp) A is a major constituent of the outer membrane of Escherichia coli. This protein has been used in several vaccine development studies, but seldom with a view to vaccinating against mastitis. The objective of this study was to investigate the immunogenicity of E. coli OmpA and its vaccine potential for cows. Both the humoral and cellular immune responses were inv...

The synthesized oligo [4-(methoxyphenyl) acetonitrile] (OMPA) was chemically modified by Knoevenagel condensation of OMPA with dimethylformamide dimethyl acetal (DMF-DMA). obtained oligomer is composed short chains poly [3-(dimethylamino)-2-(4-methoxyphenyl)acrylonitrile] and denoted OMFA. Experimental measurements: ultraviolet-visible (UV-vis), steady-state time-resolved photoluminescence spec...

introduction: acinetobacter baumannii is associated with hospital-acquired infections. outer membrane protein a of a.baumannii (abompa) is a well-characterized virulence factor which has important roles in pathogenesis of this bacterium. methods: based on our pcr-sequencing of ompa gene in the clinical isolates, abompa protein can be categorized into two types, named here type-1 and type-2. we ...

*Corresponding author. E-mail addr Abbreviations used: BSA, bovine hVDAC1, voltage-dependent aniondomain of the autotransporter NalP OmpA, outer membrane protein A o isomerases; SurA, the survival factor WT-OmpA, wild type OmpA; YaeT The interactions of outer membrane proteins (OMPs) with the periplasmic chaperone Skp from Escherichia coli are not well understood. We have examined the binding o...

The mechanism of action of Salmonella enterotoxin (Stn) as a virulence factor in disease is controversial. Studies of Stn have indicated both positive and negative effects on Salmonella virulence. In this study, we attempted to evaluate Stn function and its effects on Salmonella virulence. To investigate Stn function, we first performed in vitro and in vivo analysis using mammalian cells and a ...

Everted membrane vesicles were prepared from Escherichia coli cells containing either overproduced amounts (OP-membrane vesicles) or normal amounts (normal membrane vesicles) of SecY and SecE, both of which are essential components of the protein translocation apparatus. The rates of translocation of pro-OmpA were similar in the two types of membrane vesicles, whereas translocation ATPase activ...

A Pseudomonas aeruginosa outer membrane protein F-deficient omega-insertion mutant strain H636, in contrast to its protein F-sufficient parent strain H103, was unable to grow on unsupplemented Proteose Peptone no. 2 broth (Difco Laboratories, Detroit, Mich.). Addition of high concentrations of NaCl, KCl, glucose, sucrose, or potassium succinate permitted growth of strain H636 at rates approachi...

BACKGROUND The effectiveness of PCR methods to amplify rickettsiae from clinical samples has still not been evaluated. Our aim was to determine the sensitivity and usefulness for Rickettsia species identification by PCR methods, targeting 16S rDNA, htrA, gltA, ompA, and ompB genes for molecular diagnosis of rickettsioses. METHODS A total of 72 clinical samples (EDTA-blood, skin biopsies and t...