The NS3 protein of Japanese encephalitis virus (JEV) contains motifs typical of RNA helicase/NTPase but no RNA helicase activity has been reported for this protein. To identify and characterize the RNA helicase activity of JEV NS3, a truncated form of the protein with a His-tag was expressed in Escherichia coli and purified. The purified JEV NS3 protein showed an RNA helicase activity, which wa...

Backgroundː Numerous in vivo human cohort studies have suggested that the apolipoprotein B100/apolipoprotein AI (ApoB100/ApoAI) ratio might be a risk factor in coronary heart disease. The aim of this study was to measure ApoB100/ApoAI changes cell secretions by incubating HepG2 cells with various amounts glucose vitro.<ns...

Infections with Hepatitis C virus (HCV) pose a serious health problem worldwide. In this study, the hypothesis that adoptive transfer of dendritic cells (DCs) pulsed with HCV NS3 protein and matured with an oligodeoxynucleotide (ODN) containing CpG motifs (CpG) ex vivo would initiate potent HCV-specific protective immune responses in vivo was tested. NS3 protein was efficiently transduced into ...

Background: Surface roughness is one of the most important characteristics endodontic instruments, correlating to instrument fracture. The purpose this study was measure surface values these instruments before and after resin root canal preparation without previous glide path preparation, with new method. Data obtained from field emission scanning electron microscope...

Background: Exercise has been identified as an allied health strategy that can support the management of depression in older adults, yet relative effectiveness for different exercise modalities is unknown. To meet this gap knowledge, we present a systematic review and network meta-analysis randomised controlled trials (RCTs) to examine head-to-head aerobic, resistanc...

Background: Use of telephone, video and e-consultations is increasing. These can make consultations more transactional, potentially missing patients’ concerns. This study aimed to develop a complex intervention address concerns comprehensively in general practice test the feasibility this cluster-randomised framework. The used two technologies:<ns3:bol...

Background COVID-19 continues to pose a significant risk human health and is prominent global vampire. diseases have similar burden in all parts of the world, but they present challenge public low- middle-income nations like Bangladesh. The primary purposes study are describe extent which has increased cost living for average Bangladeshi identify chron...

IIntroduction : Bronchiolitis is the leading cause of hospitalization in children. Estimate potentially preventable variables that impact length hospital stay are a priority to reduce costs associated with this disease. This study aims identify clinical bronchiolitis children tropical middle-income country Methods:</ns3:b...

Hepatitis C viruses (HCV) encode a helicase enzyme that is essential for viral replication and assembly (nonstructural protein 3 [NS3]). This helicase has become the focus of extensive basic research on the general helicase mechanism, and it is also of interest as a novel drug target. Despite the importance of this protein, mechanistic work on NS3 has been conducted almost exclusively on varian...

Background: Previous research has consistently shown the significant difference in outcome between cancerous and non-cancerous patients with coronavirus disease 2019 (COVID-19). However, no studies have compared clinical manifestation of COVID-19 hematologic cancers solid patients. Therefore, we analyzed hematological cancer primary worldwide through a meta-analysis ...