background: the increasing pattern of multi-drug resistant (mdr) bacteria has limited therapeutic options especially for nosocomial isolates of klebsiella pneumoniae. therefore, the aim of this study was the molecular detection of 16s rrna methylase and blactx-m-15 among k. pneumoniae strains isolated from hospitalized patients in mofid, imam hossein and taleghani hospitals. materials and metho...

the lactic acid bacteria (lab) are an industrially important group of probiotic organisms that play an important role in human health by inhibiting harmful and pathogen bacteria growth, boosting immune function, and increasing resistance to infection.  this study aimed to isolate, identify, and biologically characterize probiotic lab strains from iranian traditional dairy products. a total of 2...

A rapid, sensitive, inexpensive in situ hybridization technique, using 30-mer 16S rRNA probes, can specifically differentiate two closely related Bacillus spp., B. polymyxa and B. macerans. The 16S rRNA probes were labeled with a rhodamine derivative (Texas Red), and quantitative fluorescence measurements were made on individual bacterial cells. The microscopic fields analyzed were selected by ...

Although the applicability of small subunit ribosomal RNA (16S rRNA) sequences for bacterial classification is now well accepted, the general use of these molecules has been hindered by the technical difficulty of obtaining their sequences. A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16S rRNA or cloning of its gene. The 16S rRNA...

Two recently published but independently derived structures, namely the X-ray crystallographic structure of ribosomal protein S7 and the "binding pocket" for this protein in a three-dimensional model of the 16S rRNA, have been correlated with one another. The known rRNA-protein interactions for S7 include a minimum binding site, a number of footprint sites, and two RNA-protein crosslink sites o...