Purpose: This study determined total surface energy distributions using an Inverse Gas Chromatography (IGC) to understand the de-agglomeration of fine lactose powders commonly used in dry powder inhaler formulations. Methods: The particle size distribution (PSD) of two lactose samples, Lactohale 300 (Lac A) and micronized lactose (Lac B), were determined by laser diffraction in liquid media, an...

Lung cancer is the leading cause of cancer-associated mortality worldwide. MicroRNAs (miRNAs/miRs) serve a role in the occurrence and development of lung cancer. The aim of the present study was to analyze the expression and function of the proliferation-associated miR-383-5p in lung adenocarcinoma (LAC). Samples of human LAC and matched adjacent normal lung tissues were surgically removed, and...

The use of nanomaterials allows the design of ultrasensitive biosensors with advantages in the detection of organic molecules. Catechol and catechin are molecules that occur naturally in fruits, and their presence in products like dyes and wines affects quality standards. In this study, catechol and catechin were measured at the nanoscale by means of cyclic voltammetry. The oxidation of Coriolo...

The paradoxical correlation between thrombosis and the lupus anticoagulant (LAC) effect is an enigmatic feature of the antiphospholipid (aPL) syndrome. The Dutch authors previously reported that thrombosis-related anti– 2-glycoprotein I ( 2GPI) antibodies recognize domain I and cause LAC. The American authors reported that aPLs disrupt an anticoagulant annexin A5 (AnxA5) crystal shield. We inve...

The tetrameric Lac repressor can bind simultaneously to two lac operators on the same DNA molecule, thereby including the formation of a DNA loop. We investigated the phasing dependence of DNA loop formation between lac operator O1 and an auxiliary ideal lac operator (O(id)) on the bacterial chromosome, with inter-operator distances varying from 57.5 to 1493.5 bp. Repression of a CAP-independen...

Arduini et al. raise interesting issues related to mechanisms involving carnitine (1). The authors ask whether there is a free-carnitine deficiency in Flinders Sensitive Line rats (FSL) (2) and, more broadly, raise the question of whether the deficiency of acetyl-L-carnitine (LAC) occurs systemically or in the brain in FSL (1). We found that carnitine acetyltransferase (CrAT) mRNA levels in the...

Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), J. A. Wohlhieter, R. V. Citarella, and L. S. Baron. Transfer of episomic elements to Proteus. II. Nature of lac(+)Proteus strains isolated from clinical specimens. J. Bacteriol. 88:1598-1601. 1964.-Strains of Proteus mirabilis exhibiting the unusual property of utilizing lactose (lac(+)) have been reported in clinical m...

We have shown that it is possible to screen for penicillinase-producing Neisseria gonorrhoeae directly from primary culture plates. Experiments involving the cocultivation of four genera of beta-lactamase-positive (beta-lac(+)) bacteria and a beta-lactamase-negative (beta-lac(-)) N. gonorrhoeae on modified Thayer-Martin medium indicated that suppressed or inhibited beta-lac(+) bacteria did not ...

Lupus anticoagulants (LAC) consist of antiphospholipid antibodies, detected via their anticoagulant properties in vitro. Strong LAC relate to thromboembolic events, a hallmark of the antiphospholipid syndrome. We have analyzed whether detection of this syndrome would benefit from thrombin generation measurements. Therefore, calibrated automated thrombography was done in normal plasma (n = 30) a...