Hemagglutination inhibition, enzyme-linked immunosorbent assay (ELISA), and passive latex agglutination were used to test 495 human serum samples for determination of rubella immunity. Overall agreements for immune status were as follows: hemagglutination inhibition versus ELISA, 94.7% (469 of 495); hemagglutination inhibition versus passive latex agglutination (1:10 dilution), 99.2% (491 of 49...

Since the original demonstration of the hemagglutination induced by influenza virus,, 118 a number of other important viruses have been reported as causing the agglutination of chicken erythrocytes. Specific agglutination of these red blood cells has been observed with vaccinia virus,21 the virus of Newcastle disease,1 that of fowl plague,17 variola virus,22 and mumps virus.'6 It has also been ...

A sensitive immunoassay procedure for the detection of the thrombopoietic-stimulating factor (TSF) has been developed. This procedure Is similar to the hemagglutination-inhibition (HAl) technique that is available for erythropoietin. For the production of TSF, sheep were made thrombocytopenic by the Injection of antiplatelet serum. TSF from sheep sera was partially purified by column chromatogr...

Erythrocytes collected from 1-day-old chicks were stabilized by fixation with formaldehyde and by freeze-drying after treatment with carbon monoxide. Suspensions of freeze-dried erythrocytes in distilled water or physiological saline had a homogeneous bright reddish-purple color. Freeze-dried erythrocytes were compared with fresh erythrocytes for hemagglutination and hemagglutination-inhibition...

Nonspecific inhibitors of rubella hemagglutination can be removed by treatment of sera with heparin-manganous chloride for use in the hemagglutination-inhibition test. After removal of nonspecific inhibitors by this procedure, an excess of manganous chloride may remain. This may cause the cells to agglutinate, thus obscuring the reading at low serum dilutions. This disadvantage can be overcome ...