The potato cyst nematode Globodera rostochiensis invades roots of host plants where it transforms cells near the vascular cylinder into a permanent feeding site. The host cell modifications are most likely induced by a complex mixture of proteins in the stylet secretions of the nematodes. Resistance to nematodes conferred by nucleotide-binding-leucine-rich repeat (NB-LRR) proteins usually resul...

The potato cyst nematode, Globodera rostochiensis, is an important pest of potato. Like other pathogens, plant parasitic nematodes are presumed to employ effector proteins, secreted into the apoplast as well as the host cytoplasm, to alter plant cellular functions and successfully infect their hosts. We have generated a library of ORFs encoding putative G. rostochiensis putative apoplastic effe...

Received for publication 15 July 1985. t Nematologist, Istituto Nematologia Agraria, CNR, via Amendola 165/A, 70126 Bari, Italy. Nematologist, Servicio Agricola y Ganadero, Division Protection Agricola, Ministerio de Agricultura, Santiago, Chile. s Nematologist, Division of Plant Industry, P.O. Box 1969, Gainesville, FL 32602. cliophora arenaria Raski, Longidorus spp., Paratrichodorus spp., Tri...

Much progress has been made in developing techniques for characterizing population dynamics of nematodes. Nevertheless, most assay procedures have numerous inherent limitations (1,13). The efficiency of given methods may be dependent on soil type, moisture, and other environmental and biological factors (1,13). Certain extraction methods (e.g., Baermann funnel) depend on nematode motility. In a...

The potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis are major pests of potatoes. The G. pallida (and G. rostochiensis) life cycle includes both diapause and quiescent stages. Nematodes in dormancy (diapause or quiescent) are adapted for long-term survival and are more resistant to nematicides. This study analysed the mechanisms underlying diapause and quiescence. The effects ...

Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-E...

Globodera infestation was evaluated in soils of private farm plots Chernihiv and Kyiv regions Ukraine 2017–2018. Soil samples were taken at 88 farms, 15.02 ha total, 11 settlements 6 districts. The nematode abundance the examined areas graded as follows: less than 1000 eggs + larvae/100 cm3 — low rate infestation; 1000–5000 average rate; more 5000 high rate. 85.4 % study area is infested with g...