All tRNA(His) possess an essential extra G(-1) guanosine residue at their 5' end. In eukaryotes after standard processing by RNase P, G(-1) is added by a tRNA(His) guanylyl transferase. In prokaryotes, G(-1) is genome-encoded and retained during maturation. In plant mitochondria, although trnH genes possess a G(-1) we find here that both maturation pathways can be used. Indeed, tRNA(His) with o...

The identification of flowering plants using DNA barcoding proposed in last decades has slowly gained ground Africa, where it been successfully used to elucidate the systematics and ecology several plant groups, understand their evolutionary history. Existing inferences on effectiveness identify African trees are mostly based lowland forests, whereas adjacent montane forests significantly diffe...

The genus Centaurea s.l. is one of the richest and most taxonomically complex Asteraceae family. It includes between 400 700 species, which more than 70 are found in Bulgaria – a territory considered as secondary centers speciation genus. There large number endemics (Bulgarian Balkan) with endemism reaching up to 50% some groups, such Cyanus gr. Due ongoing active Balkans, boundaries closely re...

Prostanthera eungella B.J.Conn & K.M.Proft (Lamiaceae) is described as a new species from the South Kennedy botanical region of northern Queensland. Concatenated nuclear (ETS) and chloroplast (trnH-psbA) sequence data for 38 species of Prostanthera were analysed using maximum-likelihood and Bayesian-inference methods. These combined data recovered P. eungella as part of a polytomy that included...

Methods This study uses maximum-likelihood and Bayesian phylogenetic analyses of chloroplast DNA (atpB–rbcL, trnL–trnF, trnS–trnG, psbA–trnH) and nuclear ribosomal DNA [internal transcribed spacer (ITS) and external transcribed spacer (ETS)] from 24 out of c. 37 ingroup taxa, beast molecular dating, and the dispersal–extinction–cladogenesis method (Lagrange) to reconstruct ancestral geographica...

Three chloroplast regions, rbcL, psbA-trnH and petA-psbJ were applied to assess the genetic relationships among six Curcuma medicinal species, which are difficult to distinguish from morphology. The Maximum Parsimony tree was conducted by Kimura 2-parameter model with MEGA 4. The genetic relationships were linked with geographical distributions among these six species; Curcuma sichuanensis is a...