DNA replication and its connection to M phase restraint are studied extensively at the level of single cells but rarely in the context of a developing animal. C. elegans lin-6 mutants lack DNA synthesis in postembryonic somatic cell lineages, while entry into mitosis continues. These mutants grow slowly and either die during larval development or develop into sterile adults. We found that lin-6...

OBJECTIVE To assess the relative risk of major congenital malformation (MCM) from in utero exposure to antiepileptic drug (AEDs). METHODS Prospective data collected by the UK Epilepsy and Pregnancy Register were analysed. The presence of MCMs recorded within the first three months of life was the main outcome measure. RESULTS Full outcome data were collected on 3607 cases. The overall MCM r...

Deregulation of mini-chromosome maintenance (MCM) proteins is associated with genomic instability and cancer. MCM complexes are recruited to replication origins for genome duplication. Paradoxically, MCM proteins are in excess than the number of origins and are associated with chromatin regions away from the origins during G1 and S phases. Here, we report an unusually wide left-handed filament ...

of FPGA and MCM Technology Joel Darnauer, Tsuyoshi Isshiki, Por rio Garay, John Ramirez, Vijayshri Maheshwari, Wayne Wei-Ming Dai Applied Sciences Building, Computer Engineering, UC Santa Cruz, Santa Cruz, CA 95064 Internet: @ce.ucsc.edu Abstract Multichip module technology can be used to dramatically increase the capability and performance of eld programmable gate arrays (FPGAs) and the eld pr...

The Vapnik-Chervonenkis (VC) dimension measures the complexity of a learning machine, and a low VC dimension leads to good generalization. The recently proposed Minimal Complexity Machine (MCM) learns a hyperplane classifier by minimizing an exact bound on the VC dimension. This paper extends the MCM classifier to the fuzzy domain. The use of a fuzzy membership is known to reduce the effect of ...

Introduction In recent years, a master chemical mechanism (MCM) has been developed to describe the atmospheric degradation of VOC in the atmosphere (Jenkin et al., 1997a; Saunders et al., 1997). The MCM has been developed as part of a collaborative project between the University of Leeds, AEA Technology plc and the UK Meteorological Office, and funded by the UK Department of the Environment (no...

The minichromosome maintenance complex (MCM) proteins are required for processive DNA replication and are a target of S-phase checkpoints. The eukaryotic MCM complex consists of six proteins (MCM2-7) that form a heterohexameric ring with DNA helicase activity, which is loaded on chromatin to form the pre-replication complex. Upon entry in S phase, the helicase is activated and opens the DNA dup...

Synthesis of mesoporous materials with a bimodal pore size has always been of great importance for size-selective separation process. Especially, ordered mesoporous cubic MCM-48 with its highly interwoven and branched pore structure, is a potential separation material in applications similar to which zeolites are now being used. This material could have specific technological advantages because...

We exploit an improved mammalian cell-free DNA replication system to analyse quiescence and Cdc6 function. Quiescent 3T3 nuclei cannot initiate replication in S phase cytosol from HeLa or 3T3 cells. Following release from quiescence, nuclei become competent to initiate semiconservative DNA replication in S phase cytosol, but not in G0 phase cytosol. Immunoblots show that quiescent cells lack Cd...