Salmonella spp. producing extended-spectrum beta-lactamases (ESBLs) have been reported in many countries, but there is no information on their prevalence in Africa. ESBL-producing Salmonella enterica serotype Isangi and S. enterica serotype Typhimurium strains have been noted in South Africa since 2001. A total of 160 consecutive isolates of Salmonella spp. were collected from 13 hospitals loca...

PURPOSE The complexity of sarcoma has led to the need for patient selection via in vivo biomarkers. Tumor endothelial marker-1 (TEM-1) is a cell surface marker expressed by the tumor microenvironment. Currently MORAb-004 (Ontuxizumab), an anti-TEM-1 humanized monoclonal antibody, is in sarcoma clinical trials. Development of positron emission tomography (PET) for in vivo TEM-1 expression may al...

The clinical strain Escherichia coli TO799 was resistant to penicillin-clavulanate combinations and ceftazidime and was not reproducibly detected as an extended-spectrum -lactamase (ESBL) according to the standards of the Clinical Laboratory Standards Institute (CLSI; formerly NCCLS) and the national guidelines of the French Society for Microbiology (Comité de l’Antibiogramme de la Société Fran...

a facile mechanochemical-based method for synthesis of copper oxide (cuo) nanoparticles is here by introduced. for this purpose, copper hydroxide powder was synthesized through a facile solution method (cuso4 + 2 na(oh) → cu(oh)2 + na2so4) after which milling of as-prepared cu(oh)2 precursor and nacl resulted in the mechanochemical dehydration of cu(oh)2 and dispersion of cuo nanoparticles into...

molecular sieves mcm-41 were synthesized from rice husk ash (rha) as alternative sources of silica. the material was synthesized by a room temperature method from a gel with the molar composition sio2: 1.6ea: 0.215hdtmabr: 325h2o with ph correction. the cetyltrimethylammonium bromide (ctmabr) was used as a structure template. nanocomposite containing 5.0 and 10.0 wt.% of cu was synthesized unde...

in modern medical processes, new antimicrobial drugs are being widely used in order to rapid and essential eradication of resistant bacterial strains. broad-spectrum beta-lactamases are enzymes originated from mutations in the common plasmid genes mediators for beta-lactamases such as tem-1, tem-2, and shv-1 are transferred among bacterial species. the genes coding the production of esbl are of...

This letter is meant to make scientists aware of the proper application of transmission electron microscopy (TEM) for the assessment of polymeric self-assemblies. Cryogenic (cryo)-TEM should be the method of choice. Here, we show the difference in morphologies observed in the same sample when using cryo-TEM and when using TEM with drying, demonstrating the importance of choosing the proper method.

We passaged cells expressing TEM-1 and TEM-12 from a single plasmid through either ampicillin or ceftazidime. We found that the combined effects of recombination and selection removed the bla(TEM-1) allele from the bacterial population when it was passaged through ceftazidime or the bla(TEM-12) allele when cultures were passaged through ampicillin.