Modification of aldose reductase (AR) by the nitrosothiols S-nitroso-N-acetyl penicillamine (SNAP) and N-(beta-glucopyranosyl)-N(2)-acetyl-S-nitrosopenicillamide (glyco-SNAP) resulted in a 3-7-fold increase in its k(cat) and a 25-40-fold increase in its K(m) for glyceraldehyde. In comparison with the native protein, the modified enzyme was less sensitive to inhibition by sorbinil and was not ac...

G-protein-coupled receptor activation is generally analyzed under equilibrium conditions. However, real-life receptor functions are often dependent on very short, transient stimuli that may not allow the achievement of a steady state. This is particularly true for synaptic receptors such as the α(2A)-adrenergic receptor (α(2A)-AR). Therefore, we developed a fluorescence resonance energy transfe...

The objective of the present study was to test the hypothesis that endogenous beta(3)-adrenoreceptor (AR) activation contributes to left ventricular (LV) and cardiomyocyte dysfunction in heart failure (CHF). Stimulation of the beta(3)-AR inhibits cardiac contraction. In the failing myocardium, beta(3)-ARs are upregulated, suggesting that stimulation of beta(3)-ARs may contribute to depressed ca...

multiple sclerosis (ms) is an autoimmune inflammatory process that affects the central nervous system (cns). celiac disease (cd) is a systemic autoimmune disorder of gluten intolerance. based on the presumed association of ms with multiple autoimmune processes, the coincidence of ms with gluten sensitivity has been investigated with controversial results. here, we report a known case of ms with...

The ozonation of synthetic wastewater containing azo dye Acid Red 14 (AR 14) was investigated in a high-throughput microporous tube-in-tube microchannel reactor. The effects of design and operating parameters such as micropore size, annular channel width, liquid volumetric flow rate, ozone-containing gas volumetric flow rate, initial pH of the solution and initial AR 14 concentration on decolor...

(In vitro culture of Phyllanthus stipulatus (Euphorbiaceae)). An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus stipulatus (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication rates (8-9 shoots per explant) was achieved on MS media supplemented with either 2.5-5.0 μM IBA. The best basal media for axillary shoot prol...