A putative synthetic vaccine for foot-and-mouth disease (FMDV15) has proved less successful in a host species, cattle, than predicted by results in a small-animal model. Possible reasons for this include non-recognition by T cells influenced by major histocompatibility complex (MHC)-linked immune response gene control. It is now possible to type for human leucocyte antigen (HLA) DR-like bovine ...

Nadeem Ali-Khan, Hsin-Yao Tang, Lynn Echan and David W. Speicher The wide range of protein concentrations found in human serum poses a considerable challenge with regard to proteomic analysis, as it restricts the detection and quantitation of low abundance proteins, including cancer biomarkers. A critical component of several alternative protein profiling strategies designed to detect lower abu...

Analysis by means of two-dimensional gel electrophoresis (IEF) of [32P]orthophosphate-labeled proteins from mitotic and interphase transformed amnion cells (AMA) has shown that keratins IEF 31 (Mr = 50,000; Hela protein catalogue number), 36 (Mr = 48,500), 44 (Mr = 44,000), 46 (Mr = 43,500), as well as vimentin (IEF 26; Mr = 54,000) are phosphorylated above their interphase level during mitosis...

The modern edible fungi study in China was originated in Shanghai. The Edible Fungi Institute (EFI) under the Shanghai Academy of Agricultural Science has made many breakthroughs in the Chinese edible fungi study. EFI has succeeded in getting pure spawns, has diversified the cultivation varieties of edible fungi by domesticating wild mushrooms, and has developed the bag cultivation technology. ...

Alzheimer's disease (AD) sometimes presents with prominent executive dysfunction and associated prefrontal cortex atrophy. The impact of such executive deficits on episodic memory performance as well as their neural correlates in AD, however, remains unclear. The aim of the current study was to investigate episodic memory and brain atrophy in AD patients with relatively spared executive functio...

Subunit components of R-phycoerythrins (R-PEs) prepared from five marine macro red algae were analyzed by sodium dodecyl sulfate -polyarylamide gel electrophoresis (SDS-PAGE) and by isoelectric focusing (IEF) in pH gradients range of 3.0 to 9.5, 2.5 to 5.0 and 4.0 to 6.5. Riboflavin was used to catalyze polymerization of IEF gel in acidic pH gradients, and ethanolamine and HEPES were selected a...

Although the internal electric field (IEF) of bismuth oxyiodide (BiOI) is acknowledged as a potent driving force for efficient charge separation, enhancing intensity IEF remains challenge. Herein, highly crystalline BiOI nanosheets with I-rich surface are employed to intensify and direct migration. In comparison I-poor nanosheets, which possess Bi−O layer termination I-defects, demonstrates 62....

A total of 281 Staphylococcus aureus strains selected from those submitted to the Centers for Disease Control for phage typing between 1956 and 1982 were tested for the production of toxic-shock-associated protein (TSAP) by isoelectric focusing (IEF) and solid-phase radioimmunoassay. The results suggest that the observed temporal trends in the incidence of toxic-shock syndrome were not primaril...

A more than 20000-fold purification of human serum lipoamidase is described. This was accomplished by (NH4)2SO4 precipitation and chromatography on DEAE-Sepharose, Blue Sepharose CL-6B and phenyl-Sepharose CL-4B, followed by preparative isoelectric focusing (IEF) and finally by gel-permeation chromatography. Co-precipitation and co-chromatography of lipoamidase and biotinidase activities with e...