We describe a new method of synthesizing photolabile hydrogel materials for convenient photoimmobilization of biomolecules on surfaces or in 3-D matrixes. Dissolved agarose was modified with photolabile S-(2-nitrobenzyl)cysteine (S-NBC) via 1,1'-carbonyldiimidazole (CDI) activation of primary hydroxyl groups. S-NBC-modified agarose remained soluble and gelable with up to 5% S-NBC substitution, ...

The naive credal classifier (NCC) extends naive Bayes classifier (NBC) to imprecise probabilities to robustly deal with the specification of the prior; NCC models a state of ignorance by using a set of priors, which is formalized by Walley’s Imprecise Dirichlet Model (IDM). NCC has been shown to return more robust classification than NBC. However, there are particular situations (which we preci...

Oligodendrocytes (OLs) are cells that produce myelin in the central nervous system. Here we use ratiometric pH indicator dye to analyze intracellular pH in OLs in culture. The results reveal alkaline microdomains, which predominate in the perikaryon and proximal dendrites, and acidic microdomains, which predominate in distal dendrites. Spatial nonuniformity of pH is generated by differential su...

Abstract Various copolymers incorporated with β-substituted acrylates, such as alkyl crotonates (e.g., methyl crotonate (MC), ethyl (EC), isopropyl (iPC), and n -butyl (nBC)) cinnamate (MCin), were synthesized by group-transfer polymerization (GTP) using a silicon-based Lewis acid catalyst. In addition to α-substituted acrylates methacrylate (MMA) (nBMA)) examined comonomers. Proton nuclear mag...

The objective of this study was to analyze the effect of bleaching toothpastes, both conventional and those containing the new whitening agent Blue Covarine, on teeth previously bleached by conventional techniques (in-office and at-home). Squared bovine enamel/dentin blocks (6.0 x 6.0 x 2.0 mm) were randomly distributed in 6 groups (n = 15), according to the technique used to bleach them (in-of...

Conflicts with American black bears (Ursus americanus) are increasing in rural and suburban areas throughout the United States. Human encroachment on bear range has increased availability of garbage, bird feeders, beehives, and other food sources. Preventing bears from attaining food from anthropogenic sources could mitigate these conflicts. We tested a new shocking mechanism that is being mark...

Protective clothing limits heat transfer and hampers task performance due to the increased weight. Militarism protective clothing enables humans to operate in adverse environments. In the selection and evaluation of militarism protective clothing attention should be given to heat strain, ergonomic and fit issues next to the actual protection it offers. Fifty Male healthy subjects participated i...

BACKGROUND AND PURPOSE To study the accuracy, specificity and sensitivity of the Naïve Bayesian Classifier (NBC) in the assessment of individual risk of cancer relapse or progression after radiotherapy (RT). MATERIALS AND METHODS Data of 142 brain tumour patients irradiated from 2000 to 2005 were analyzed. Ninety-six attributes related to disease, patient and treatment were chosen. Attributes...

Classification of cancer patients using traditional methods is a challenging task in the medical practice. Owing to rapid advances in microarray technologies, currently expression levels of thousands of genes from individual cancer patients can be measured. The classification of cancer patients by supervised statistical learning algorithms using the gene expression datasets provides an alternat...

The nanobiocatalyst (NBC) is an emerging innovation that synergistically integrates advanced nanotechnology with biotechnology and promises exciting advantages for improving enzyme activity, stability, capability and engineering performances in bioprocessing applications. NBCs are fabricated by immobilizing enzymes with functional nanomaterials as enzyme carriers or containers. In this paper, w...