A unique experimental model is described, where natural immunologic tolerance to a well-defined soluble native antigen (murine C5) is examined in congenic strains of mice that differ only by the presence or the absence of C5. A highly sensitive hemolytic assay was developed to detect nanogram amounts of C5 as well as an assay of anti-C5 inhibition of C5 hemolytic activity. The latter was more s...

Experimental conditions required for the expression of maximum C5 activation upon limited trypsin hydrolysis were determined to be 0.008 mol of trypsin/mol C5 in a reaction mixture containing 1 mg C5/ml veronal-buffered saline incubated at 37 degrees C for 30 min. Employing these optimal incubation conditions, the primary or preferred site of trypsin hydrolysis of the C5 alpha-chain resulted in...

Leptospirosis is considered one of the most important zoonosis worldwide. The activation of the Complement System is important to control dissemination of several pathogens in the host. Only a few studies have employed murine models to investigate leptospiral infection and our aim in this work was to investigate the role of murine C5 during in vivo infection, comparing wild type C57BL/6 (B6 C5+...

در این مقاله حل دقیق ارتعاش برون صفحه‌ای نانو ورق‌های مستطیلی نسبتاً ضخیم براساس تئوری غیر‌محلی تغییر شکل برشی سینوسی اصلاح شده با نظر گرفتن شرایط مرزی لوی ارائه است. هدف اصلی تحقیق بررسی اثر مقیاس کوچک بر پارامترهای فرکانسی می‌باشد. برای توصیف تأثیرات خارج از صفحه‌ی ورق غیر محلی ارینگن استفاده شده. مشتمل شش حالت مختلف شامل دو ضلع موازی دارای تکیه‌گاه ‌ساده و اضلاع دیگر ترکیبی ساده، گیردار آزاد ...

Complement C5-deficient (C5(-/-)) macrophages derived from B.10 congenic mice were found to be defective in killing intracellular Mycobacterium tuberculosis (MTB). They were bacteriostatic after activation with IFN-gamma alone but bactericidal in the combined presence of IFN-gamma and C5-derived C5a anaphylatoxin that was deficient among these macrophages. Reduced killing correlated with a decr...