objective: helicobacter pylori is to blame for one of the most common chronic infections in humans and can cause peptic ulcer, gastritis, duodenitis and non-ulcer dyspepsia. developing a reliable test to detect the infection is of great importance. for a variety of reasons, the existing tests including the stool culture, biopsy, pcr, and urea breath test are not up to par. the pcr-elisa test is...

background helicobacter pylori multiplies and causes infection in human gastric mucosal layer. new approaches have focused on using specific treatments, such as immunotherapy, to limit this infection. urease, as one of the most important virulent and antigenic factors of the bacterium, is a suitable target for this purpose. patients and methods in order to prepare recombinant proteins, the synt...

Ammonia production from urea by ureolytic oral bacteria is believed to have a significant impact on oral health and the ecological balance of oral microbial populations. In this study we cloned and characterized the urease gene cluster of Actinomyces naeslundii, which is one of the pioneer organisms in the oral cavity and a significant constituent of supragingival and subgingival dental plaque ...

We have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical Vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. Here, we cloned and sequenced the 15,754-bp DNA region containing the trh gene and the gene cluster for urease prod...

Background and objective: Proteus mirabilis is part of the Enterobacteriaceae family, Gram negative bacterium which typically lives in human gut, means when it causes illness can be a serious bloodstream infection, urinary tract infection or disseminated infection. UreC ZapA virulent genes constitute major pathogenicity this organism as well its resistance to antibiotics. This study was carried...