A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 microM) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was...

We investigated the regeneration of two Bulgarian sweet pepper cultivars (‘Hebar’ and ‘Stryama’) on solid and liquid culture medium. Liquid cultures were incubated in temporary immersion RITA ® airlift bioreactors. During a period of 60 days, culture in the liquid medium was associated with significantly increased shoot tip growth, number of lateral shoots, percentage of rooted plants and micro...

Micropropagation is an important technique for rapid and large-scale multiplication of selected plants. The type and concentration of plant growth regulators and physiological status of the explant have profound effect on the efficacy of plant regeneration. However, success of any micropropagation protocol depends upon the percentage survival and successful establishment of plants from in vitro...

In vitro culture of Dioscorea wightii belongs to the family Dioscoreace. An efficient micropropagation protocol was developed for the medicinal plant Dioscorea wightii using nodal segments for axillary shoot proliferation. Callus initiation was stimulated at the basis of the explants in 100% of the cultures by 0.15-1.75μM BA, 0.75-5.0μM Kinetin, 0.15-0.30μM 2iP.The average fresh mass of calluse...

Micropropagation of Ilex dumosa var. dumosa R. ("yerba señorita") from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in 1/4 strength Murashige and Skoog medium (1/4 MS) plus 30 gr x L(-1) sucrose and supplemented with 4.4 microM BA. Further multiplication and elongation of the regenerated shoots were obt...

Background Conifer clonal forestry as a form of plantation forestry has great potential advantages. However, vegetative propagation of conifers, especially that of mature trees, is quite problematic. These species also are most recalcitrant objects for cultivation in vitro. For this reason the development of an effective system for clonal micropropagation of some conifers is still an important ...

Wetland grasses and grass-like monocots are very important natural remediators of pollutants. Their genetic improvement is an important task because introduction of key transgenes can dramatically improve their remediation potential. Tissue culture is prerequisite for genetic manipulation, and methods are reported here for in vitro culture and micropropagation of a number of wetland plants of v...