Two different honey bee subspecies’ genotypes obtained from Ordu, Apiculture Research Institute were analyzed by SNP markers using Real-Time PCR-HRM. Genomic DNA samples analysed with 10 primers those used for identification of chalkbrood disease resistance genes and two genom sequencing. Result analyses, four (AMB-00858574, AMB-01151447, AMB00631190, AMB-00686140) amplified in Caucasian six AM...

Cynara scolymus L., known as globe artichoke, is a medicinal plant widely used in food supplements (PFS) and herbal infusions due to its beneficial health properties. The high demand for artichoke-containing products can lead adulteration practices. In this work, real-time polymerase chain reaction (PCR) system coupled high-resolution melting (HRM) analysis was proposed differentiate C. from ot...

BACKGROUND The staphylococcal protein A (spa) locus of Staphylococcus aureus contains a complex repeat structure and is commonly used for single-locus sequence-based genotyping. The real-time PCR platform supports genotyping methods that are single step and closed tube and potentially can be carried out simultaneously with diagnosis. We describe here a method for genotyping S. aureus using high...

Background: Candida albicans is the predominant yeast reported from human infection. Non-albicans species have been recently developed as medically vital fungi. Therefore, it essential to detect and identify pathogens at level prescribe appropriate treatment. Methods: This study assessed two complementary methods, including real-time polymerase chain reaction-high resolution melt (PCR-HRM) reac...

background: the aim of the current study was to assess the mrna levels of two mitochondria-related genes, including nuclear-encoded nrf1 (nuclear respiratory factor 1), mitochondrial transcription factor a (tfam), and mitochondrial-encoded cytochrome c oxidase subunit 1 (mt-co1) genes in various stages of the human oocyte maturation. methods: oocytes were obtained from nine infertile women with...

background and objectives: we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. materials and methods: the specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. in total, 193 clinical isolates were ...