In this perspective, we discuss the cytoplasm in actively growing bacterial cells contrasted with viable but nonculturable (VBNC) cells. Actively growing bacterial cells contain a more molecularly crowded and organized cytoplasm, and are capable of completing their cell cycle resulting in cell division. In contrast, nutrient starving bacteria in the physiological VBNC state are struggling to su...

Citrobacter freundii strain WA1 was stressed by incubation in seawater microcosms for eleven years. After two years of starvation, no culturable strain was observed. Incubation of samples in nutrient-rich broth medium not supplemented with growth factors, however, allowed resuscitation of VBNC cells so that subsequent plating yielded observable colonies for significantly extended periods of tim...

Pseudomonas syringae infects diverse crop plants and comprises at least 50 different pathovar strains with different host ranges. More information on the physiological and molecular effects of the host inhibitory environment on the pathogen is needed to develop resistant cultivars. Recently, we reported an in vitro model system that mimics the redox pulse associated with the oxidative burst in ...

Like many other gram-negative bacteria, the human pathogen Vibrio vulnificus is induced into a viable but nonculturable (VBNC) state by incubation at low temperatures. The ability of any bacterium to resuscitate from this dormant state would appear to be essential if the VBNC state is truly a survival strategy. The question as to whether the culturable cells which appear following removal of th...

The aim of this study was to evaluate the survival responses of two strains of Vibrio splendidus, both in natural and in defined media. For this purpose, freshwater and defined media containing different salinities (3.3-0.9 %) and nutrient concentrations (17-0.005 mg x l(-1)) were assayed. The incubation temperatures were established at 4, 10 and 22 degrees C. The acridine orange staining techn...

Yersinia pestis, the causative agent of plague, has caused several pandemics throughout history and remains endemic in the rodent populations of the western United States. More recently, Y. pestis is one of several bacterial pathogens considered to be a potential agent of bioterrorism. Thus, elucidating potential mechanisms of survival and persistence in the environment would be important in th...

Abstract Background Managing surgical site infections, with negative culture report in routine diagnosis is a common dilemma microbiology accounting more than 30% worldwide. The present study attempted to identify the presence of bacterial spp. if any wound aspirates/swabs infections hospitalised patients using molecular tools. Methods Ninety-seven post-operative SSI whose swabs/aspirate were c...

Many species of bacteria enter a viable but non-culturable (VBNC) state when exposed to stressful conditions. The VBNC acts as an adaptive strategy that allows for long-term survival in unfavorable environments. are difficult detect the environment due inability grow on nutrient agar. Although ability may be advantageous bacteria, it poses risk human health. Francisella tularensis, transitions ...

An issue of critical concern in microbiology is the ability to detect viable but nonculturable (VBNC) and viable-culturable (VC) cells by methods other than existing approaches. Culture methods are selective and underestimate the real population, and other options (direct viable count and the double-staining method using epifluorescence microscopy and inhibitory substance-influenced molecular m...