introduction: glycemia and blood glucose levels are under the effect of hb a1c and therefore they could be considered as a valid index for the follow-up and control of diabetes .there are different and controversial reports on the effects of iron deficiency anemia on hb a1c. effects of ida in non diabetic patients are also unknown. the aim of the study was the comparison of hb a1c levels in hea...

Solution and cell studies were performed to ascertain why individuals with hemoglobin (Hb) SC have disease whereas those with Hb AS do not. The polymerization of deoxygenated mixtures containing sickle cell Hb (Hb S; alpha 2 beta 2(6)Glu leads to Val) and Hb C (alpha 2 beta 2(6)Glu leads to Lys) was investigated by measurements of delay times and solubilities. In mixtures containing more than 4...

To clarify the role of gammaN-terminal Gly, gamma5 Glu, and gamma143 Ser in 2,3-biphosphosphoglycerate (BPG) binding to fetal hemoglobin (Hb F), we engineered and produced normal human Hb F and two Hb F variants (Hb F gammaG1V, gammaS143H, and Hb F gammaG1V, gammaE5P, gammaS143H) using a yeast expression system and then compared their oxygen-binding properties with those of native human Hb F an...

background: hemoglobin (hb) and hematocrit (hct) have been used routinely in the diagnosis of neonatal anemia and polycythemia.   objective: to measure the cord blood hb and hct and to determine their association with maternal hb and hct.   methods : 170 healthy neonates born through normal vaginal delivery with gestation age of 37-42 wks and birth weight of 2.5-4 kg, and their mothers were inc...

Human hemolysate contains several minor components designated Hb A,,, Hb Alh, Hb A,,, which are post-translational modifications of the major hemoglobin component A,,. Individuals with diabetes mellitus have elevated levels of Hb A,,, a hemoglobin modified with a glucose moiety at the NH, terminus of each /3 chain. A new chromatographic technique using Bio-Rex 70 is described which not only all...

To further understand determinants for Hemoglobin (Hb) S polymerization, as well as the inhibitory mechanism of Hb F on Hb S polymerization, Hb F variants containing Val-* (Hb F yE6V) or Val-*, Thr-y87 (Hb F yE6V. yQ87Tj were expressed in yeast. The oxy form of Hb F yE6V was about 10-fold less stable to mechanical agitation than native oxy Hb F, which is similar to stability differences compari...