The primary objective of this study was to examine the quality volunteered geographic information (VGI) data for flood mapping Hurricane Harvey. As a crowdsourcing platform, U-Flood project mapped flooded streets in Houston metro area. This research examines following: (1) If there are any significant differences water depth (WD) among hydraulic and hydrologic (H&H) model, Federal Emergency...

Staphylococci are the leading cause of nosocomial blood stream infections. Fast and accurate identification of staphylococci and confirmation of their methicillin resistance are crucial for immediate treatment with effective antibiotics. A multiplex real-time PCR assay that targets mecA, femA specific for S. aureus, femA specific for S. epidermidis, 16S rRNA for universal bacteria, and 16S rRNA...

We propose the use of DNA microarray for the discrimination of homologous products after a single PCR amplification with consensus primers. The method was applied to Staphylococcus identification. The femA nucleotide sequences, which are phylogenetically conserved among the staphylococci, were first amplified using a consensus primer pair together with the mecA sequence, a molecular marker for ...

Floods continue to inflict the most damage upon human communities among all natural hazards in the United States. Because localized flooding tends to be spatially repetitive over time, local decisionmakers often have an opportunity to learn from previous events and make proactive policy adjustments to reduce the adverse effects of a subsequent storm. Despite the importance of understanding the ...

Peptidoglycan (PG) composition in intact cells of methicillin-resistant Staphylococcus aureus (MRSA) and its isogenic Fem mutants has been characterized by measuring the glycine content of PG bridge structures by solid-state nuclear magnetic resonance (NMR). The glycine content estimated from integrated intensities (rather than peak heights) in the cell walls of whole cells was increased by app...

The role of higher-“mode” pushover analyses in seismic analysis of buildings is examined in this paper. It is demonstrated that the higher-“mode” pushover curves reveal plastic hinge mechanisms that are not detected by the first-“mode” or other FEMA-356 force distributions, but these purely local mechanisms are not likely to develop during realistic ground motions in an otherwise regular buildi...

HURRICANE KATRINA WAS THE MOST DEVASTATING natural disaster in US history. Large parts of New Orleans and nearby Louisiana parishes were destroyed. About 90 000 square miles of the Gulf Coast, an area roughly the size of Great Britain, was declared a federal disaster area. The often contaminated flood waters covering much of New Orleans for almost 2 months contained a mix of raw sewage, bacteri...