The plasmid pGKE75-catA138T, which comprises pUC18 and the catA138T gene encoding thermostable chloramphenicol acetyltransferase with an A138T amino acid replacement (CATA138T), serves as an Escherichia coli-Geobacillus kaustophilus shuttle plasmid that confers moderate chloramphenicol resistance on G. kaustophilus HTA426. The present study examined the thermoadaptation-directed mutagenesis of ...

The first TCR-dependent checkpoint in the thymus determines αβ versus γδ T lineage fate and sets the stage for later T cell differentiation decisions. We had previously shown that early T cells in NOD mice that are unable to rearrange a TCR exhibit a defect in checkpoint enforcement at this stage. To determine if T cell progenitors from wild-type NOD mice also exhibit cell-autonomous defects in...

An R-phycoerythrin (R-PE) was isolated by gel filtrations on Sepharose CL-4B and Sephadex G-150 from the phycobiliprotein extract of the marine red macroalga Polysiphonia urceolata Grev and further purified by ion exchange chromatography on DEAE-Sepharose Fast Flow. The purified R-PE showed three absorption peaks at 498 nm, 538 nm, 566 nm and one fluorescent emission maximum at 577 nm. Although...

A preconditioned AOR iterative method is proposed with the preconditioner I + S∗ αβ. Some comparison theorems are given when the coefficient matrix of linear system A is an irreducible L−matrix. The convergence rate of AOR iterative method with the preconditioner I + S∗ αβ is faster than the convergence rate with the preconditioner I + Sα by Li et al. Numerical example verifies comparison theor...

The association of desmin with the α-crystallin Β-chain (αΒ-crystallin; encoded by CRYAB), and the fact that mutations in either one of them leads to heart failure in humans and mice, suggests a potential compensatory interplay between the two in cardioprotection. To address this hypothesis, we investigated the consequences of αΒ-crystallin overexpression in the desmin-deficient (Des-/-) mouse ...

A preconditioned AOR iterative method is proposed with the preconditioner I + S∗ αβ. Some comparison theorems are given when the coefficient matrix of linear system A is an irreducible L−matrix. The convergence rate of AOR iterative method with the preconditioner I + S∗ αβ is faster than the convergence rate with the preconditioner I + Sα by Li et al. Numerical example verifies comparison theor...

The vacuum solution ds 2 = dx 2 + x 2 dy 2 + 2 dz dt + ln x dt 2 of the Einstein gravitational field equation follows from the general ansatz ds 2 = dx 2 + g αβ (x) dx α dx β but fails to follow from it if the symmetric matrix g αβ (x) is assumed to be in diagonal form.

It is well known that a majorana mass induces a (small) transition magnetic moment. The converse is also true; in this paper we estimate the loop contribution of transition magnetic moments [μ]αβ to the neutrino mass matrix [m]αβ . We show that for hierarchical neutrino masses, the contribution of [μ]eτ to [m]eτ can exceed the experimental value of [m]eτ .

The αβ associative memories recently developed in Ref. 10 have proven to be powerful tools for memorizing and recalling patterns when they appear distorted by noise. However they are only useful in the binary case. In this paper we show that it is possible to extend these memories now to the gray-level case. To get the desired extension, we take the original operators α and β, foundation of the...