At least 8 macromolecules in the supernatant fraction of rat liver immunologically cross-react with antigens extracted from liver microsomes. These supernatant analogs of microsomal antigens have been resolved by extensive column electrophoresis and differentially characterized. One species («)is highly acidic, 3 (ß, TI, and y2) are of intermediate acidity, and 4 (*, ei, 62>and Õ)are near-ne...

The possible mitogenic activity of fibronectin (FN) in human primary and metastatic melanoma lines and clones and the involvement of integrins in mediating this effect were evaluated. Quiescent human melanoma cells cultured in serum-free medium proliferated in a doseand timedependent fashion to immobilized FN as indicated by [3H]thymidine incorporation, increment of cell number, and cell cycle ...

The effects of various cytostatic agents (mechlorethamine, 1-/3-D-arabinofuranosylcytosine, 6-mercaptopurine, methotrexate, colchicine, vincristine, a podophyllotoxin derivative, bleomycin, 1,2-bis(3,5-dioxopiperazin-1-yl)propane, the epipodophyllotoxin derivatives 4'-O-demethyl-1O-[4,6-O-2-thenylidene-ß-D-glucopyranosyl]epipodophyllotoxin and 4'-O-demethyl-1-O-[4,6-O-ethylidene-/3-D-glucopyra...

Vincristine concentration in serum from 1 min to 72 hr was measured by radioimmunoassay in 14 patients with cancers following i.v. bolus injection of Vincristine sulfate at 0.45 to 1.30 mg/sq m. The pharmacokinetic data were analyzed by a nonlinear least-square regression program NONLIN. A threecompartmental open model fitted the raw data better than a two-compartmental model. The mean half-liv...

We have applied restriction fragment length polymorphism analysis to a 30-member panel of primary glioma DNAs, which had been previ ously examined for loss of genetic information (C. D. James, E. Carlbom, J. P. Dumanski, M. Hansen, M. Nordenskjold, V. P. Collins, and W. K. Cavenee, Cancer Res., 48:5546-5551, 1988), to determine the frequency and sublocalization of loss of genetic information fr...

Two murine models, C3H 38C13 B-cell lymphoma and AKR SL2 I cell lymphoma were used to determine the efficacy of three different Interferon preparations, recombinant human hybrid interferon-a A/D, recombinant murine Interferon (rMIFN)-7, and natural MIFN-a//3 (>85% ß), alone and in combination with tumor specific and nonspecific monoclonal antibody therapy. All three Interferon preparations hav...

Human bladder cancer from cystoscopic biopsies and from established cell lines was transplanted into mice that were immune suppressed by thymectomy plus sequential treatment with 1-ß-0-arabinofuranosylcytosine and whole-body irradia tion. Each of four established human bladder cancer cell lines generated transplantable tumors in these mice, and some of the mice developed pulmonary métastases....

The effects of ovine interstitial cell-stimulating hormone (ICSH), ovine prolactin, or bovine growth hormone (BGH) on the growth and fine structure of transplantable Leydig cell tumors have been investigated. The Leydig cell tumor was capable of growth in the hypophysectomized mouse as well as in the intact mouse treated with rabbit antisera to ICSH. The ultrastructure of the Leydig cell was al...

Using a modification of the collagenase dispersion method of Dufau et a/., we examined changes in DMA synthesis produced by estrogens in the interstitial cells of mice that develop malignant Leydig cell tumors after prolonged estrogen administration. Previous work in cryptorchid mice indicated that during continuous estrogen administration ['H]thymidine incor poration into DNA rises to a maximu...

The complementarity determining region III of the rearranged iiniminoglobulin heavy chain gene has been the target for tumor-specific PCR assays for the detection and follow-up of B-cell malignancies. Previously, these assays have relied on gel-based end point data collection methods (i.e., band densitometry) and, thus, have provided at best a semiquanti tative assessment of tumor levels. We sh...