The study of zinc finger proteins has revealed their potential to act as oncogenes or tumor suppressors. Here we report the molecular, biochemical, and functional characterization of KS1 (KRAB/zinc finger suppressor protein 1), a novel, ubiquitously expressed zinc finger gene initially isolated from a rat pancreas library. KS1 contains 10 C2H2 zinc fingers, a KRAB-A/B motif, and an ID sequence ...

Targeted manipulation of complex genomes often requires the introduction of a double-strand break at defined locations by site-specific DNA endonucleases. Here, we describe a monomeric nuclease domain derived from GIY-YIG homing endonucleases for genome-editing applications. Fusion of the GIY-YIG nuclease domain to three-member zinc-finger DNA binding domains generated chimeric GIY-zinc finger ...

Sequence-specific DNA binding has been demonstrated for a synthetic peptide comprising only one of the two "finger"-like domains of the erythroid transcription factor GATA-1 (also termed Eryf-1, NF-E1, or GF-1). Quantitative analysis of gel-retardation assays yields a specific association constant of 1.2 x 10(8) M, compared with values of about 10(9) M for the full-length natural GATA-1 protein...

nloaded transcription factor NF-E2-related factor 2 (Nrf2) binds the antioxidant DNA response element (ARE) to te important cellular cytoprotective defense systems. Recently several types of cancers have been shown rexpress Nrf2, but its role in the cellular response to radiation therapy has yet to be fully determined. In udy, we report that single doses of ionizing radiation from 2 to 8 Gy act...

Anonymizing network provides web services to the users and also hide the client’s IP address from the server. All data is wrapped with several layer of encryption. The success of this network, hackers can easily deface popular web site. If the users misbehave, blocking particular IP addresses is difficult. Nymble system is a credential system in which servers can blacklist misbehaving users in ...

The mechanism through which the large serine recombinases bind DNA is poorly understood. Alignments of C31 integrase (Int) and its relatives indicate the presence of a conserved motif containing four cysteines resembling a zinc finger. Inductively coupled plasma-mass spectrometry (ICP-MS) confirmed that an Int monomer contains one atom of zinc. Pre-incubation of Int with ethylenediaminetetraace...

GTPase activating proteins (GAPs) from pathogenic bacteria and eukaryotic host organisms deactivate Rab GTPases by supplying catalytic arginine and glutamine fingers in trans and utilizing the cis-glutamine in the DXXGQ motif of the GTPase for binding rather than catalysis. Here, we report the transition state mimetic structure of the Legionella pneumophila GAP LepB in complex with Rab1 and des...

The plant homeodomain (PHD) zinc finger is a structural motif of about 40-60 amino acid residues found in many eukaryotic proteins that are involved in chromatin-mediated gene regulation. The human chromodomain helicase DNA binding protein 4 (CHD4) is a multi-domain protein that harbours, at its N-terminal end, a pair of PHD finger motifs (dPHD) connected by a ~30 amino acid linker. This tandem...

The interaction among concurrently executing threads of a program results in insidious programming errors that are difficult to reproduce and fix. Unfortunately, the problem of verifying a concurrent boolean program is undecidable [24]. In this paper, we prove that the problem is decidable, even in the presence of unbounded parallelism, if the analysis is restricted to executions in which the n...