Methods: Blood, urine, and stool samples were collected from 687 hospitalized children with diarrhoea in Basrah, Iraq. The causative agents (Bacteria and parasites) for diarrhoea were identified, especially E.coli O157: H7 which was subjected to serotyping by fluorescent technique. Direct smear; Ritchie formalin– ether–sedimentation concentration and modified Ziehl-Neelsen staining techniques w...

to investigate the prevalence of cryptosporidium infection in horses, a total of 221 faecal specimenswere collected from 18 villages of urmia, northwestern iran. the samples were studied microscopically bymodified ziehl-neelsen staining. the cryptosporidial infection was found in the collected samples from 11 of18 villages. although the prevalence of the infection was 15.8%, none of the infecte...

Microscopy as “field based test” was compared with IS900 blood PCR for the diagnosis and estimationof prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) in domestic livestock withclinical Johne’s disease (JD). Of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood PCR, respectively. Proportional agreement between “microscopy” and blood PCR was subst...

The Ziehl-Neelsen stain is compared with three alternative methods of staining tubercle bacilli in paraffin sections: Fite's method (1938); a modification by Armstrong and Price (1947) of Fite's method; and a fluorescent method using the auramine-phenol stain. The fluorescent method was found to be the most effective of the four methods and its use is recommended as a routine tool in diagnostic...

Materials and Methods: A total of 510 different extra pulmonary samples were collected during the period of August 2014 to July 2015. All the samples were subjected to Ziehl Neelsen staining. Samples were decontaminated by NALC-NaOH method and inoculated on a pair of Lowenstein-Jensen (LJ) media and one p-Nitrobenzoic acid (PNB). Cultures were incubated at 37 ̊C and observed for 8weeks for any g...

We report a case of pulmonary nocardiosis in an immunosuppressed patient having vasculitis who presented with fever, cough and chest pain. A suspicion of nocardiosis was made on auramine O staining of material procured by CT guided fine needle aspiration cytology right lung. Modified Ziehl-Neelsen staining was useful in confirming the diagnosis. The patient showed remarkable recovery after trea...

To evaluate Ziehl-Neelsen (ZN) staining using variants of carbol-fuchsin solution, duplicate smears from 416 samples were stained with ZN, one set with 1% basic fuchsin and the other 0.3%. Another set of duplicate smears from 398 samples were stained with ZN, one with 1% basic fuchsin and the other 0.1%. The coded smears were read and discrepancies resolved. All samples underwent mycobacterial ...

ABSTRACT Mycobacteriosis was detected in seven out of one Atlantic mackerel (Scomber scombrus) that purchased for human consumption from a fish market. The apparently healthy but during cleaning, several granulomatous foci were noticed the visceral organs. Histopathological examination lesions revealed numerous characterized by caseous necrosis center lesion surrounded epithelioid giant cells. ...

To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from pati...