In addition to controlled expression of genes by specific regulatory circuits, the abundance proteins and transcripts can also be influenced physiological states cell such as growth rate metabolism. Here we examine control gene metabolism, analyzing a multi-omics dataset consisting absolute-quantitative abundances transcriptome, proteome, amino acids in 22 steady-state yeast cultures. We find t...

The yeast Saccharomyces cerevisiae CDC25 gene encodes a nucleotide-exchange-factor (NEF) that can convert the inactive GDP-bound state of RAS proteins to an active RAS-GTP complex. CDC25 can activate the yeast RAS proteins as well as the human H-ras protein. CDC25 is a member of a family of yeast genes that likely encode NEFs capable of regulating the RAS-related proteins found in yeast. By ali...

The results presented here are part of a study of pentose synthesis in Alcaligenes fuecalis. The mechanism of pentose synthesis is of interest since the organism can synthesize pentose from acetate, but cannot use hexoses for growth (1). In the course of the investigation into the enzymes associated with this synthesis, glyceraldehyde phosphate dehydrogenase activity, with either triphosphopyri...

Yeast Saccharomyces cerevisiae contains two genes, RAS1 and RAS2, which show remarkable homology to mammalian ras genes. To characterize these gene products, we have expressed the RAS2 gene in yeast using an inducible GAL10 promoter. After labeling with [35S]methionine and immunoprecipitating with a monoclonal antibody Y13-259, which reacts with p21 encoded by mammalian ras genes, a major band ...

In the yeast Saccharomyces cerevisiae, DNA polymerase zeta (Polzeta) is required in a major lesion bypass pathway. To help understand the role of Polzeta in lesion bypass, we have performed in vitro biochemical analyses of this polymerase in response to several DNA lesions. Purified yeast Polzeta performed limited translesion synthesis opposite a template TT (6-4) photoproduct, incorporating A ...

All histidine tRNA molecules have an extra nucleotide, G-1, at the 5' end of the acceptor stem. In bacteria, archaea, and eukaryotic organelles, G-1 base pairs with C73, while in eukaryotic cytoplasmic tRNAHis, G-1 is opposite A73. Previous studies of Escherichia coli histidyl-tRNA synthetase (HisRS) have demonstrated the importance of the G-1:C73 base pair to tRNAHis identity. Specifically, th...

We report the identification and purification of a yeast factor functionally homologous to the human upstream element factor (UEFh). Although the yeast protein (UEFy) has a higher molecular weight than the HeLa UEF (60 kD versus 45 kD) both have identical DNA-binding properties: the purified UEFy recognizes the Adenovirus 2 (Ad2) major late promoter upstream element (MLP-UE; from nucleotide -49...

A number of trace nucleotides are reported to be present in ribonucleic acids from various sources. Cohn (I) and Davis and Allen (2) have described a nucleotide in yeast ribonucleic acids, the nucleoside of which has recently been identified as 5ribosyluracil.’ Littlefield and Dunn (3) have found small amounts of thymine, 2-methyladenine, N6-methyladenine2 (6methylaminopurine), and Ne-dimethyla...