نتایج جستجو برای: xylanase hydrolysis
تعداد نتایج: 40520 فیلتر نتایج به سال:
Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain
Abstract Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis important step in a biorefinery context. This research evaluated the synergism among four classes hydrolytic purified enzymes—endo-1,4-?-xylanase, ?- l -arabinofuranosidase, ?-xylosidase, d -glucuronidase—over glucuronoar...
Wheat straw is a highly promising raw material for bio-refinery strategies. Most of the literature related to lignocellulose fractionation focuses on cellulose purification and hemicellulose solubilization. Pre-treatments solubilization without formation undesired products usually reach low extraction yields, which leaves an important fraction unused. In this work, we propose mild process effic...
Accellerase 1000 cellulase, Spezyme CP cellulase, β-glucosidase, Multifect xylanase, and beta-xylosidase were evaluated for hydrolysis of pure cellulose, pure xylan, and switchgrass solids from leading pretreatments of dilute sulfuric acid, sulfur dioxide, liquid hot water, lime, soaking in aqueous ammonia, and ammonia fiber expansion. Distinctive sugar release patterns were observed from Avice...
Xylanases (E.C.3.2.1.8) are key enzymes, which play an important role in the breakdown of xylan. Wheat bran and corn cob are a rich source of xylan (28%) and xylose (23%). Therefore these are attractive substrates for production of xylanase & ßxylosidase enzyme. Xylan, a major component of hemicelluloses, is a heterogeneous polysaccharides consisting of ß-1, 4 linked to D-xylosyl residues on th...
The present work deals with the development of solid state fermenter for enzymes production and the development of a pilot scale of enzymatic deinking system. The newly developed solid state fermenter, was named FERMSOSTAT®, which was used for the production of cellulases and xylanase using a local isolate; Aspergillus niger USM AI 1. Optimization of enzymes production was carried out and the o...
Holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. Combinations of crude enzymatic extracts from different fungal species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger and Trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton res...
Solids resulting from pretreatment of corn stover by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid, lime, and sulfur dioxide (SO(2)) technologies were hydrolyzed by enzyme cocktails based on cellulase supplemented with beta-glucosidase at an activity ratio of 1:2, respectively, and augmented with up to 11.0 g xylanase protein/g cellulase protein ...
BACKGROUND Improving the hydrolytic performance of xylanolytic enzymes on arabinoxylan is of importance in the ethanol fermentation industry. Supplementation of debranching (arabinofuranosidase) and depolymerizing (xylanase) enzymes is a way to address the problem. In the present study, we identified a bifunctional α-l-arabinofuranosidase/endo-xylanase (Ac-Abf51A) of glycoside hydrolase family ...
An extracellular thermo-alkali-stable and cellulase-free xylanase from Geobacillus thermodenitrificans A333 was purified to homogeneity by ion exchange and size exclusion chromatography. Its molecular mass was 44 kDa as estimated in native and denaturing conditions by gel filtration and SDS-PAGE analysis, respectively. The xylanase (GtXyn) exhibited maximum activity at 70 °C and pH 7.5. It was ...
Background Using globally abundant crop residues as a carbon source for energy generation and renewable chemicals production stand out as a promising solution to reduce current dependency on fossil fuels. In nature, such as in compost habitats, microbial communities efficiently degrade the available plant biomass using a diverse set of synergistic enzymes. However, deconstruction of lignocellul...
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