The microbial fuel cell (MFC) is an ecologically friendly alternative energy source. Due to the typically limited electron transfer in MFC systems, co-biocatalysts are necessary enhance their performance. Enzymes used as due superior ability generate energy, and system known enzymatic (EMFC). One of substrates that may be bagasse waste extracted from sugarcane. Saccharo­myces cerevisiae enzyme ...

Access to chiral alcohols of high optical purity is today frequently provided by the enzymatic reduction of precursor ketones. However, bioreductions are complicated by the need for reducing equivalents in the form of NAD(P)H. The high price and molecular weight of NAD(P)H necessitate in situ recycling of catalytic quantities, which is mostly accomplished by enzymatic oxidation of a cheap co-su...

Lactobacillus brevis alcohol dehydrogenase (Lb-ADH) catalyzes reduction of prochiral carbonyl compounds to chiral alcohol and meanwhile consumes its cofactor NADH into NAD(+), while the cofactor regeneration can be catalyzed by Candida boidinii formate dehydrogenase (Cb-FDH). This work presents three different Escherichia coli whole-cell biocatalyst systems expressing recombinant ADH/FDH, FDH-L...

BACKGROUND Whole cell-catalyzed biotransformation is a clear process option for the production of chiral alcohols via enantioselective reduction of precursor ketones. A wide variety of synthetically useful reductases are expressed heterologously in Escherichia coli to a high level of activity. Therefore, this microbe has become a prime system for carrying out whole-cell bioreductions at differe...

background: recent studies indicate an increased incidence of pertussis disease in recent years. the aim of this study was to evaluate the efficacy of the acellular vaccine for children (as a replacement of current whole cell vaccine in the expanded program on immunization) and for high-risk adults in iran through updating current best available evidence.   methods: we performed a systematic li...

Microbially derived surfactants, so-called biosurfactants, have drawn much attention in recent years and are expected to replace current petrochemical surfactants, owing to their environmental and toxicological benefits. One strategy to support that goal is to reduce production costs by replacing relatively expensive sugars with cheaper raw materials, such as short-chain alkanes. Herein, we rep...

Abstract Scaling up biocatalytic reactions involving cytochrome P450 monooxygenases (P450s) is challenging due to their instability, low substrate loading, co‐factor, and oxygen requirements as well the dependency on a reductase partner, which limits integration into synthetic chemistry. Recently, study investigated frequently used bacterial P450s produce bioactive stilbenoids, extending repert...