The satellite tobacco necrosis virus RNA is uncapped and requires a 3' translational enhancer domain (TED) for translation. Both in the wheat germ extract and in tobacco, TED stimulates in cis translation of heterologous, uncapped RNAs. In this study we investigated to what extent translation stimulation by TED depends on binding to wheat germ factors. We show that in vitro TED binds at least s...

Trichomoniasis is one of the most common parasitic sexually transmitted diseases in the world. Metronidazole was known as the most effective drug for human trichomoniasis., however, drug resistance and toxicity appeared. This study was designed to investigate the in vitro inhibitory activity of wheat germ agglutinin and Nigella sativa aqueous extract on the growth and motility of Trichomonas va...

We have analyzed ligand-receptor complexes resulting from (i) the incubation of canine hepatic plasma membranes with [125I]iodoglucagon and (ii) subsequent gentle solubilization of receptor-bound ligand with digitonin. The complexes (molecular weight approximately equal to 500,000) retain the radiolabeled ligand during gel filtration and subsequent manipulation at 4 degrees C in the absence of ...

INTRODUCTION Cardiovascular diseases (CVD) are the leading cause of mortality worldwide and diet is an important contributor to CVD risk. Thus, several food derivatives are being investigated for their beneficial impact on reducing cardiometabolic risk factors, either in risk groups or in healthy population as a preventive measure. Wheat germ is a food by-product with high nutritional value, es...

Sialic acid is believed to play a critical role in the survival of blood platelets in circulation. Wheat germ agglutinin, which shows specificity for sialic acid, N-acetylglucosamine, and N-acetylgalactosamine, strongly activates platelets. The role of sialic acid in platelet activation by this lectin was studied utilizing neuraminidase-treated platelets and the succinylated lectin that has bee...

Antisera were raised against P3 (mol. wt. 32 000), the full-length translation product of alfalfa mosaic virus (A1MV) RNA 3. P3 was made by translation in wheat germ extracts, using unfractionated AlMV RNA as message, and the products were then fractionated either by centrifugation through a sucrose gradient, or by phosphocellulose chromatography, each technique being followed by SDS-PAGE. Prep...

The sulfated glycosaminoglycans, such as keratan sulfate and chitin sulfate having 3-hydroxy free N-acetyl-beta-D-glucosaminyl residues as constituents, reacted with wheat germ agglutinin and Solanum tuberosum agglutinin by sugar-specific interaction. The glycosaminoglycans showed different inhibitory activities to the hemagglutination reaction of these lectins and keratan sulfate and its modif...