It is well established that test statistics and P-values derived from discrete data, such as genetic markers, are also discrete. In most genetic applications, the null distribution for a discrete test statistic is approximated with a continuous distribution, but this approximation may not be reasonable. In some cases using the continuous approximation for the expected null distribution may caus...

Using singular perturbation methods, the existence and stability of traveling wave solutions for a density-dependent selection migration model in population genetics is proved. Single locus and two alleles are assumed, and it is also assumed that the fitnesses of the heterozygotes in the population are close to but below those of the homozygotes. Unlike previous models, this paper does not assu...

this study was conducted to investigate the single nucleotide polymorphism of stat1 gene by pcr-rflp technique and association of genotypes with milk production traits in brown swiss dairy cattle. the blood samples of ninety eight cows were collected randomly from tail vein. the dna was extracted with cinagene kit (pr881612c). fragment of 314 bp of stat1 gene was amplified by pcr and digested b...

This paper addresses the issue of exact-test based statistical inference for Hardy-Weinberg equilibrium in the presence of missing genotype data. Missing genotypes often are discarded when markers are tested for Hardy-Weinberg equilibrium, which can lead to bias in the statistical inference about equilibrium. Single and multiple imputation can improve inference on equilibrium. We develop tests ...

background : to determine the frequency of the single nucleotide polymorphism m287t in exon 9 of the as3mt gene in iranian population and to assess the difference in allele frequencies with other ethnicities. subjects and methods : genotyping analysis was performed on 150 healthy subjects using the pcr-rflp assay. we used chi-square analysis to check the deviation from hardy–weinberg equilibriu...

Standard statistical tests for equality of allele frequencies in males and females and tests for Hardy-Weinberg equilibrium are tightly linked by their assumptions. Tests for equality of allele frequencies assume Hardy-Weinberg equilibrium, whereas the usual chi-square or exact test for Hardy-Weinberg equilibrium assume equality of allele frequencies in the sexes. In this paper, we propose ways...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study, the polymorphism of the cast gene of makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (pcr–sscp). genomic dna was extracted from whole blood samples collected from 100 sheep. a 622 bp cast exon 1 segmen...

We successfully cloned the Wistar-Imamichi (WI) rat growth hormone gene (GenBank accession: GQ890681), which contained 5 exons and 4 introns. Using the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) technique, a novel missense substitution single nucleotide polymorphism was identified and tested for Hardy-Weinberg equilibrium with the χ(2) test. This gene fragment ...

the pituitary-specific transcription factor (pit1) gene is responsible for pituitary development and hormone secreting gene expression in mammals. pit-1 is studied as a candidate genetic marker for growth, carcass and also for milk yield traits. in dairy farm animals, the principal goal of the selection is the improvement of milk yield and composition. the genes of milk proteins and hormones ar...