نتایج جستجو برای: vp1 protein

تعداد نتایج: 1235758  

2012
Yi-Yang Lien Chi-Hung Huang Fang-Chun Sun Shyang-Chwen Sheu Tsung-Chi Lu Meng-Shiunn Lee Shu-Chin Hsueh Hsi-Jien Chen Meng-Shiou Lee

Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd...

Journal: :Journal of virology 2009
Peggy P Li Noriko Itoh Marika Watanabe Yunfan Shi Peony Liu Hui-Jung Yang Harumi Kasamatsu

Proper folding of newly synthesized viral proteins in the cytoplasm is a prerequisite for the formation of infectious virions. The major capsid protein Vp1 of simian virus 40 forms a series of disulfide-linked intermediates during folding and capsid formation. In addition, we report here that Vp1 is associated with cellular chaperones (HSP70) and a cochaperone (Hsp40) which can be coimmunopreci...

Journal: :Molecular & cellular proteomics : MCP 2010
Glen K Shoemaker Esther van Duijn Sue E Crawford Charlotte Uetrecht Marian Baclayon Wouter H Roos Gijs J L Wuite Mary K Estes B V Venkataram Prasad Albert J R Heck

Viral capsid assembly, in which viral proteins self-assemble into complexes of well defined architecture, is a fascinating biological process. Although viral structure and assembly processes have been the subject of many excellent structural biology studies in the past, questions still remain regarding the intricate mechanisms that underlie viral structure, stability, and assembly. Here we used...

Journal: :Virus research 2016
Caroline Ross Nicole Upfold Garry A Luke Özlem Tastan Bishop Caroline Knox

The VP1 subunit of the picornavirus capsid is the major antigenic determinant and mediates host cell attachment and virus entry. To investigate the localisation of Theiler's murine encephalomyelitis virus (TMEV) VP1 during infection, a bioinformatics approach was used to predict a surface-exposed, linear epitope region of the protein for subsequent expression and purification. This region, comp...

Journal: :Acta biochimica Polonica 2016
Jingyue Ma Yuan Liu Yuanjun Liu Lingjie Li Shuping Hou Xibo Gao Manli Qi Quanzhong Liu

Chlamydia phage PhiCPG1 has been found in Chlamydia caviae in a guinea pig model for inclusion conjunctivitis, raising the possibility that Chlamydia phage is also present in patients infected with C. trachomatis (Ct). In the present study, we assayed for presence of Chlamydia phage capsid protein VP1 genes and antibodies in 84 non-Ct controls and 206 Ct patients using an enzyme-linked immunoas...

Journal: :The Journal of infectious diseases 2011
Leonid Gorelik Carl Reid Manuela Testa Margot Brickelmaier Simona Bossolasco Annamaria Pazzi Arabella Bestetti Paul Carmillo Ewa Wilson Michele McAuliffe Christopher Tonkin John P Carulli Alexey Lugovskoy Adriano Lazzarin Shamil Sunyaev Kenneth Simon Paola Cinque

Progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease caused by JC virus (JCV) infection of oligodendrocytes, may develop in patients with immune disorders following reactivation of chronic benign infection. Mutations of JCV capsid viral protein 1 (VP1), the capsid protein involved in binding to sialic acid cell receptors, might favor PML onset. Cerebrospinal fluid seq...

2015
Milda Norkiene Jomante Stonyte Danguole Ziogiene Egle Mazeike Kestutis Sasnauskas Alma Gedvilaite

BACKGROUND Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly into virus-like particles (VLPs). As cell culturing systems for HPyV are current...

Background: Foot-and-mouth disease (FMD) is a highly contagious and economically important disease that affects cloven-hoofed animals worldwide. In recent years, a series of outbreaks of FMD have occurred in many countries. Recombinant protein synthesis incorporating protective B- and T-cell epitopes are candidates for new safer and more effective (FMD) vaccines that have potential to provide p...

2013
Mei Shi Yaping Zhou Limin Cao Cuijun Ding Yun Ji Qinbo Jiang Xiping Liu Xiang Li Xueling Hou Hongjun Peng Weifeng Shi

The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 18...

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