نتایج جستجو برای: very low density lipoprotein
تعداد نتایج: 2037295 فیلتر نتایج به سال:
We describe a simple system for the preparation of a hyperlipidemic control serum. Beta- and pre-beta-lipoproteins are removed from the serum and prepared as a stable diluent; the extracted serum is then lyophilized. Upon addition of the lipoprotein diluent, which was observed to contain only liproprotein and sodium bicarbonate, the serum reconstitutes rapidly, usually within 5 min. By a suitab...
We have previously shown that sex and obesity independently affect basal very low density lipoprotein (VLDL)-triglyceride (TG) kinetics. In the present study, we investigated the effect of hyperglycemia-hyperinsulinemia on VLDL-TG kinetics in lean and obese men and women (n = 6 in each group). VLDL-TG kinetics were measured during basal, postabsorptive conditions and during glucose infusion (5....
The cause of corticosteroid-induced hyperlipoproteinemia was studied in rats and mice. An ultrastructural morphometric method was utilized to demonstrate alterations in hepatocyte very low density lipoprotein content, and Triton WR 1339-treated rats were used to identify changes in the removal of very low density lipoproteins from plasma. The results show that corticosteroid treatment results i...
The previously described procedure of direct determination of LDL-cholesterol after selective extraction of VLDL and HDL with polycations and a polycation-exchange resin has been improved by using dodecoylated poly(ethyleneimine) instead of poly(ethyleneimine). The acyl derivative has a higher affinity for lipoproteins. Disc-electrophoretic patterns of lipoproteins separated by this procedure c...
Lipid levels are increasing in all age groups in the Chinese population, but the use of statin treatment in the elderly is not well documented. We examined serum lipids, statin usage and achievement of lipid goals in 3950 subjects aged ≥65 years. Established CVD was present in 7.77% of participants and increased CVD risk was common. Elevated LDL-C according to CVD risk level was present in 46.7...
Appearance, pharmacokinetics, and distribution of astaxanthin E/Z and R/S isomers in plasma and lipoprotein fractions were studied in 3 middle-aged male volunteers (37-43 years) after ingestion of a single meal containing a 100 mg dose of astaxanthin. The astaxanthin source consisted of 74% all-E-, 9% 9Z-, 17% 13Z-astaxanthin (3R,3'R-, 3R,3'S; meso-, and 3S,3'S-astaxanthin in a 1:2:1 ratio). Th...
Lipoprotein electrophoresis was performed on serum from subjects with and without coronary heart disease, and the patterns compared with the serum concentrations of triglyceride and cholesterol. The beta- and pre-beta-lipoproteins, expressed as a percentage of the total lipoprotein, correlate strongly with the cholesterol and triglyceride concentrations, respectively. The beta- and pre-beta-lip...
1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the ...
A method is described for the reliable, fast, and relatively inexpensive fractionation of plasma lipoproteins and quantitation of their cholesterol content. This procedure requires 350 microliter of plasma and can be completed within 3 h. Plasma lipoproteins (175 microliter of plasma) were prestained with Fat Red 7B and centrifuged (Beckman Airfuge) at plasma density (d = 1.006 kg/liter) and at...
There are errors in the Author Contributions. The correct contributions are: Conceived and designed the experiments: GL LX TF YC GJ HZ JW XC CW. Performed the experiments: GL LX TF JW. Analyzed the data: GL LX TF. Contributed reagents/materials/analysis tools: GL. Wrote the paper: GL. Additionally, there is an error in the legends for Fig. 1 and Fig. 2. “Table 1” should say “Table 5.” Please vi...
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