Livers from nonfasted rats were perfused in situ with concentrations of valine (plus [I-14C]valine) in the medium ranging from 0.3 to 15 m&r. The relationship of total free intracellular valine (y) to external valine (x) was of the form y = a + bx, where the slope was about 1.0 and the intercept nearly 0.4 mM. At low valine concentrations the ratio of intracellular to extracellular valine, afte...

Clostridium sporogenes has been found to require L-leucine and L-valine for growth in a minimal medium, although valine can be replaced by isobutyrate and leucine by isovalerate. Cells grown in minimal media incorporated significant 14C from [14C]valine into leucine and from [14C]leucine into valine. Growth with [4,5-3H]leucine also resulted in the incorporation of 3H into valine. These results...

Six experiments were conducted to determine the true digestible valine requirement of 5- to 20-kg pigs. In Exp. 1, a valine-deficient diet for 5- to 10-kg pigs was developed and validated in terms of growth performance in response to supplemental L-valine. A different basal diet was validated for 10- to 20-kg pigs in Exp. 2. Both diets were demonstrated to be deficient in valine and to support ...

Two experiments were carried out to evaluate the effects of digestible valine supplementation in pre-starter and starter diets on productivity, nutrient metabolizability coefficient (NMC), immune response broilers from breeders different ages. Experiments I II conducted with (1 7 days age) (8 21 phases, respectively. Broilers fed that differed their content. In each trial, 400 male Cobb [...]

Addition of L-valine (50 to 200 mM) to glucose minimal medium had no effect on the growth of wild-type Corynebacterium glutamicum ATCC 13032 but inhibited the growth of the derived valine production strain VAL1 [13032 DeltailvA DeltapanBC(pJC1ilvBNCD)] in a concentration-dependent manner. In order to explore this strain-specific valine effect, genomewide expression profiling was performed using...