A PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with specific primers for ureC gene amplification (herein referred to as ureC PCR) was compared with other routine invasive methods (culture, the rapid-urease test, and Giemsa staining of histological sections) with samples from a group of 104 consecutive dyspeptic patients. Bacteria were found in 40 (38.5%), 38 (3...

infectious diseases are one of the most common causes of morbidity and mortality and the spread of resistant microorganisms is playing a significant role in this regard. the purpose of this study was to assess the trend in antimicrobial resistance of gram-positive bacteria at the main referral teaching hospital in tehran during a 4-year period. all patients' biological isolates such as blood, u...

The paper by Shen, et al. reports on efforts to engineer the gut microbiota to treat hyperammonemia by decreasing the levels of urease positive gut bacteria.1 After treatment of mice with oral antibiotics and PEG to deplete indigenous bacteria, mice were inoculated with a consortium of 8 bacteria with low urease gene content. This new gut microbiota was relatively stable for up to 120 days. The...

Urease as a potential target of antimicrobial drugs has received considerable attention given its versatile roles in microbial infection. Development of effective urease inhibitors, however, is a significant challenge due to the deeply buried active site and highly specific substrate of a bacterial urease. Conventionally, urease inhibitors are designed by either targeting the active site or mim...

Urease is an important enzyme both in agriculture and medicine research. Strategies based on urease inhibition is critically considered as the first line treatment of infections caused by urease producing bacteria. Since, urease possess agro-chemical and medicinal importance, thus, it is necessary to search for the novel compounds capable of inhibiting this enzyme. Several computational methods...

The increased use of chemical fertilizer input in agricultural production and the promotion sustainable agriculture encourage researchers around globe to undertake experiments regarding application organic fertilizers on tomato production. This study aims amalgamate comprehensive effects compared with pure (100% CF) soil properties, yield, fruit quality through meta-analysis. It helps provide a...

BACKGROUND Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein. RESULTS Most alpha...

Bacterial chemical reactions, such as urea hydrolysis can induce calcium carbonate precipitation. The induced production of formed by microorganisms has been widely used in environmental and engineering applications. present study aimed to isolate, identify optimize growth conditions urease positive bacteria from rich soil Gaza Strip. isolates, which tolerated ≥10% concentration, were select...