نتایج جستجو برای: upstream and downstream fragments from target gene and antibiotic resistance cassette from plasmid pet28a

تعداد نتایج: 17827133  

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده زبانهای خارجی 1392

the present study was an attempt to compare the effect of peer metalinguistic corrective feedback on elementary and intermediate efl learners speaking ability to see which level benefits more from this type of feedback. to this end, 117 female efl learners at grade 3, al-zahra high school in kermanshah, iran were non-randomly chosen. the homogeneity of the participants was attained through a pi...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سیستان و بلوچستان - دانشکده مهندسی عمران 1391

today, scouring is one of the important topics in the river and coastal engineering so that the most destruction in the bridges is occurred due to this phenomenon. whereas the bridges are assumed as the most important connecting structures in the communications roads in the country and their importance is doubled while floodwater, thus exact design and maintenance thereof is very crucial. f...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز - دانشکده ادبیات و زبانهای خارجی 1392

abstract i the purpose of this study was to launch a thorough investigation concerning the possibility of differing orientations to the writing proficiency construct by native and non-native english speaking teacher raters. it mainly revolved around the international english language testing system (ielts) that is widely administered and employed as a measure of general proficiency in englis...

ژورنال: ارمغان دانش 2022

Introduction: In recent years, antibiotic resistance has been on the rise, leading to limited ways to control nosocomial infections (especially surgical site infections) and the right treatment options. The main purpose of this study was the pattern of antibiotic resistance and the frequency of plasmid-dependent quinolone resistance (qnr) genes in Pseudomonas aeruginosa, Escherichia coli and Kl...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز - دانشکده ادبیات و زبانهای خارجی 1391

this study was intended to analyze the listening tapescripts of the elementary and pre-intermediate levels of total english textbooks from the pragmatic dimension of language functions and speech acts in order to see whether the listening tasks are pragmatically informative or not. for this purpose, 8 conversations from the two books were selected randomly, and then, the two pragmatic models of...

Journal: :iranian journal of biotechnology 2007
hassan motejadded josef altenbuchner

a new system is presented for the generation ofrecombinant bacillus subtilis strains without antibioticmarkers. this system is based on two plasmids constructed in escherichia coli. the first plasmid phm30contains an incomplete hisi gene, the last gene in thehistidine biosynthesis operon of b. subtilis and part ofthe genes yvca and yvcb of unkown function flankinghisi at the 3´-end. the spectin...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه گیلان - دانشکده علوم انسانی 1391

short story as an important literary genre in the recent decade is focused by many of young writers and literary critical with searching structure, content , artistic techniques and cognitive phenomenon of these works , we can show quantity of using some parts of storied literature from new literal techniques of the word and also we can show the usage of native, cultural and social foundation...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه ولی عصر (عج) - رفسنجان - دانشکده ریاضی 1389

in this thesis, first the notion of weak mutual associativity (w.m.a.) and the necessary and sufficient condition for a $(l,gamma)$-associated hypersemigroup $(h, ast)$ derived from some family of $lesssim$-preordered semigroups to be a hypergroup, are given. second, by proving the fact that the concrete categories, semihypergroups and hypergroups have not free objects we will introduce t...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و علوم انسانی دکتر علی شریعتی 1393

this study aimed at examining the effects of iranian efl learners’ anxiety, ambiguity tolerance, and gender on their preferences for corrective feedback (cf, henceforth). the effects were sought with regard to the necessity, frequency, and timing of cf, types of errors that need to be treated, types of cf, and choice of correctors. seventy-five iranian efl students, twenty-eight males and forty...

Journal: :Journal of Genome Editing and Regulation 2022

CRISPR/Cas9-mediated base editing introduces point mutations in cellular DNA by exploiting target-specific single guide RNA (sgRNA) along with a genetically modified Cas9. Existing plasmid vectors for sgRNA expression contain either fluorescent marker or an antibiotic resistance cassette but not both, preventing simultaneous monitoring and enrichment of transfected host cells. In this study, we...

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