High throughput sequencing technology has great promise for biodiversity studies. However, an underlying assumption is that the primers used in these studies are universal for the prokaryotic or eukaryotic groups of interest. Full primer universality is difficult or impossible to achieve and studies using different primer sets make biodiversity comparisons problematic. The aim of this study was...

In order to study the genetic diversity in rice (Oryza sativa L.), 29 genotypes consisting land races, pure and improved lines were evaluated using simple sequence repeat (SSR) markers. A total of 30 SSR primers were used to amplify some part of rice genome in germplasms, the PIC values ranged from 0.07 (RM 340) to 0.71 (RM 7426) with an average of 0.45. The results showed a total number of 106...

Several site-directed mutagenesis methods have been developed and are being currently used in research. All these methods apply three or four primers, including one or two bearing the appropriate mutation, and most use PCR to amplify the desired sequence and to incorporate the mutation (1–6). Although several developments have improved the robustness and reliability of the method, all of them r...

This study evaluated the effect of dentin surface treatment on shear bond strengths of two light- cured glass ionomer restorative materials to dentin. Cylinders of Fuji II LC and VariGlass VLC were bonded to dentin surfaces that were untreated, treated with a 10% polyacrylic acid (GC Conditioner), or treated with a dentin bonding agent primer (Prisma Universal Bond 3 Primer). Specimens were the...

A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in...

Aromatic (Bas-370, PB-1) and non-aromatic (Pusa-677, Pusa-834) rice were selected for the characterization and for distribution of lipoxygenase (Lox) genes. Polymorphism was observed when genomic DNA of rice varieties was hybridized with a heterologous lipoxygenase probe. A distinct polymorphic fragment (approximately 1.2 kb) was found in Bas-370. Sub-genomic library of Bas-370 was constructed ...