A reversed phase high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo C18 column (250 mm×4.6 mm, 5 μm) was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid (0.2%, v/v). Gradient elution was carried out...

A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer (pH 4.5): Isopropanol (98:2, v/v), at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detect...

Overview Purpose: To examine the use of targeted principal component analysis (PCA) with HPLC-charged aerosol detector data as a means of determining adulteration of olive oils. Method: Oil samples were analyzed as both untreated and hydrolyzed, using a C30 HPLC method and charged aerosol detection. PCA results were determined using peak area percent values of the acylglycerides and free fatty ...

The presented case study illustrates the possibility of adding value to biological surplus remaining from mushroom cultivation industry. In essence, unused parts were submitted UV-C irradiation, with purpose increasing vitamin D2 content and validating its extraction. Vitamin concentration in three different species (Agaricus bisporus, A. bisporus Portobello, Pleurotus ostreatus) was obtained b...

High-performance liquid chromatography (HPLC) was used for the determination of compounds occurring during the production of biodiesel from karanja and jatropha oil. Methanol was used for fast monitoring of conversion of karanja and jatropha oil triacylglycerols to fatty acid methyl esters and for quantitation of residual triacylglycerols (TGs), in the final biodiesel product. The individual sa...

The screening and quantitation of methamphetamine (MP) in urine using dansyl chloride (DNC) as the derivatization reagent were studied. Urinary MP derivatized with DNC could be detected by visual observation of the fluorescence in a solid-phase extraction column such as a Sep-Pak C18 cartridge to which the whole reaction solution was applied. The DNC-derivatized MP was eluted from the cartridge...

Ezetimide belongs to a class of lipid lowering    compounds    that    selectively    inhibits intestinal   absorption   of   cholesterol   and   related phytosterols. The purpose of this study is to establish a reliable and quick method for the assignment of ezetimibe in tablets form b...

Analysis of fructose and glucose from glucose isomerization process using immobilised glucose isomerase (IGI), {Sweetzyme from Novozymes} are often performed by HPLC methods with refractive index (RI) detector. This study is focused on developing new methods of measuring glucose and fructose using a specific carbohydrate column. The importance of this research, primarily based on the performanc...

the present study was designed to develop a simple, validated liquid chromatographic method for the analysis of azithromycin in bulk and pharmaceutical dosage forms using ultraviolet detector. the best stationary phase was determined as c18 column, 5 µm, 250 mm × 4.6 mm. mobile phase was optimized to obtain a fast and selective separation of the drug. flow rate was 1.5 ml/min, wavelength was se...

Ethylenediaminetetraacetic acid (EDTA) is a chemical that harmful to human health with its high solubility in water, which used as metal chelating agent industries such medicine, food, personal care product, agriculture. Thus, it necessary should be monitored surface waters taken from dams supplying drinking and utility water. This work presents the applicability of HPLC-UV/VIS system for quant...