A total of 341 fertilized and 37 unfertilized oocytes from 63 intracytoplasmic sperm injection (ICSI) treatment cycles were included for retrospective assessment using the Embryoscope time-lapse video system. The second polar body (pb2) extrusion occurred at 2.9±0.1 h (range 0.70-10.15 h) relative to sperm injection. All oocytes reduced in size following sperm injection (p<0.05) with shrinkage ...

This study evaluates the effectiveness of using crushed construction and demolition waste as reactive media for applying in Permeable Reactive Barriers to treat contaminated groundwater by landfill leachate. Modern analytical methods such SEM EDX were used analyze physicochemical properties waste, demonstrating that material has a porous structure on surface is suitable thetargeted heavy metal ...

The production of biosorbents by waste biomass has attracted considerable attention due to the low cost and abundance raw materials. Here biochar produced from Eucalyptus camdulensis sawdust (EU-biochar) via pyrolysis at 600 °C was used as a potential biosorbent for Ni2+ Pb2+ metal ions wastewater. Characterization experiments indicated formation C- O-bearing functional groups on EU-biochar sur...

The present study was carried out to investigate the adsorption and leaching behavior of Cu2+, Zn2+ and Pb2+ by sediments collected from the western banks of three different sectors along River Nile at Aswan governorate, Egypt. The feasibility of sediments for the removal of Cu2+, Zn2+ and Pb2+ from aqueous solutions was tested under the effect of three conditions (pH, initial metal concentrati...

The RNA genome of influenza A viruses is transcribed and replicated by the viral RNA-dependent RNA polymerase, composed of the subunits PA, PB1, and PB2. High-resolution structural data revealed that the polymerase assembles into a central polymerase core and several auxiliary highly flexible, protruding domains. The auxiliary PB2 cap-binding and the PA endonuclease domains are both involved in...

In the last hundred years, four influenza pandemics have been experienced, beginning with that in Spain in 1918. Influenza A virus causes severe pneumonia and its RNA polymerase is an important target for drug design. The influenza A (H1N1) virus has eight ribonucleoprotein complexes, which are composed of viral RNA, RNA polymerases and nucleoproteins. PB2 forms part of the RNA polymerase compl...

H9N2 influenza viruses have been circulating worldwide in multiple avian species and have repeatedly infected humans to cause typical disease. The continued avian-to-human interspecies transmission of H9N2 viruses raises concerns about the possibility of viral adaption with increased virulence for humans. To investigate the genetic basis of H9N2 influenza virus host range and pathogenicity in m...

Dilution can best be defined as the proportion of waste tonnage to the total weight of ore and waste in each block. Predicting the internal dilution based on geological boundaries of waste and ore in each block can help engineers to develop more reliable long-term planning designs in mining activities. This paper presents a method to calculate the geological internal dilution in each block and ...

Influenza virus nucleoprotein (NP) is a critical factor in the viral infectious cycle in switching influenza virus RNA synthesis from transcription mode to replication mode. In this study, we investigated the interaction of NP with the viral polymerase protein complex. Using coimmunoprecipitation with monospecific or monoclonal antibodies, we observed that NP interacted with the RNP-free polyme...