This report demonstrates directly, using two-dimensional gel electrophoresis and alloantisera, the following: (a) The DR4 light chains show a structural polymorphism among the Dw4, DKT2, and DYT cells. (b) Most of the class II light chains consist of the DR light chain. (c) The MT3 molecule is distinct from the DR4 molecule in the Dw4, DKT2, and DYT cells. (d) The MT3 molecule does not show any...

2DE and 2D-DIGE based proteomics analysis of serum from women with endometriosis revealed several proteins to be dysregulated. A complete list of these proteins along with their mass spectrometry data and subsequent bioinformatics analysis are presented here. The data is related to "Investigation of serum proteome alterations in human endometriosis" by Dutta et al. [1].

Protein separation by two-dimensional electrophoresis (2DE) is largely used in proteomic approaches because of both high resolution and the availability of powerful image analysis software for gel comparison and compatibility with subsequent protein characterization by mass spectrometry [1]. For these various aspects, the selection of the protein staining procedure is of major importance [2]. B...

Joan-J Bech-Serra1, Núria Colomé1, ProteoRed2, Andy Borthwick3, Martin Wells3, Juan-Pablo Albar4, Francesc Canals1 1 Proteomics Laboratory Medical Oncology Research Program. Vall d’Hebron University Hospital Research Institute, Barcelona, Spain; 2ProteoRed consortium. Spanish National Institute of Proteomics; 3Nonlinear Dynamics, Newcastle, UK; 4Centro Nacional de Biotecnologia-CSIC, Madrid, Spain

Quantitative proteomic studies, based on two-dimensional gel electrophoresis, are commonly used to find proteins that are differentially expressed between samples or groups of samples. These proteins are of interest as potential diagnostic or prognostic biomarkers, or as proteins associated with a trait. The complexity of proteomic data poses many challenges, so while experiments may reveal pro...

Two-dimensional gel electrophoresis separates all of the component proteins of the ribosomal subunits of Escherichia coli. This method shows 21 proteins in the 30S, and 34 proteins in the 50S, subunit.

MOTIVATION Detection of protein spots in two-dimensional gel electrophoresis images (2-DE) is a very complex task and current approaches addressing this problem still suffer from significant shortcomings. When quantifying a spot, most of the current software applications include a lot of background due to poor segmentation. Other software applications use a fixed window for this task, resulting...

Purified antibodies from an antiserum against S-phase proteins of the myxomycete Physarum polycephalum were attached to protein-A-Sepharose CL-4B. A late G2-phase extract that contained a mitosis-stimulating protein was applied to this immunoadsorbent, and the mitosis-stimulating protein was enriched by a factor of ten. This protein, which is present in the cell in low amounts, is synthesized i...

The term proteomics was coined by Marc R. Wilkins in 1994, whilst he was developing the concept as PhD student in Macquarie University (Wilkins et al., 1996a). Proteomics was mainly derived from the field of two-dimensional gel electrophoresis (2-DE). With the arrival of the concept of proteomics or proteome analysis, the method of 2-DE has gained greater significance. Although alternative meth...