Sixty-nine positive cultures were obtained from sixty-nine specimens of sputum from practically all stages of tuberculosis. Six of these specimens were negative by direct microscopic examination, but the cultures gave positive findings. These six specimens have been positive for tubercle bacilli at some time. Nineteen positive cultures were isolated from thirty-two specimens of feces. All these...

Older observations on the nutritive requirements and metabolism of virulent tubercle bacilli do not have quantitative significance because of the lack of accurate methods for the estimation of the rate of growth. The recognition in recent years that these organism grow just as well when submerged in liquid media as on the surface of liquid or solid media has eliminated some of the difficulties ...

as part of a series of investigations at baba baghi leprosarium in lran, 44 long-treated leprosy patients were selected for our study. samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (afb), and cultured for tubercle bacilli. these tests were negative, but the polymerase chain reaction (pcr) for an insertion sequence believed to be sp...

In view of the growing interest and increased incidence of tuberculosis in India, it is extremely important to determine the various sources of dissemination of tubercle bacilli. It has been estimated that as many as 40 per cent of cattle are tuberculous in certain parts of England and about 7 per cent of samples of milk in London contain tubercle bacilli. Although milk is generally boiled in I...

Sputum culture for tubercle bacilli on a solid egg medium containing glycerol was compared with culture on a similar medium containing pyruvic acid and no glycerol. Tubercle bacilli from the sputum of five out of 99 patients grew on pyruvic acid medium but not on glycerol medium. The addition of pyruvic acid is therefore essential when an egg medium is used for the cultural diagnosis of tubercu...

BACKGROUND Tear of the Peroneus longus in association with a prominent peroneal tubercle is rare. CASE PRESENTATION Recently we treated two long distance runners who developed lateral ankle pain. Maximum tenderness was located over the lateral surface of the heel in the area of the peroneal tendons. Imaging disclosed a tear of the peroneus longus at the area of the peroneal tubercle. CONCLU...

The investigations of Sabin and her co-workerse9 demonstrated that the tubercie phosphatide was the biologically most active fraction of the various split-products of the tubercle bacillus as separated by Anderson.2 It produced epithelioid cell response and tubercle formation. Sabin also observed that, after intraperitoneal injection, the rabbit monocytes phagocytized the phosphatide, but were ...

Much progress has been made during recent years towards the identification of the components of tubercle bacilli which are responsible for tubercle formation and for the development of tuberculin allergy. I t has never been proved however that these two phenomena can account--singly or jointly--for all the systemic manifestations of tuberculosis. Indeed, there are a few facts which suggest that...

The power of a large number of aniline dyes to restrain the growth of Bacillus tuberculosis and Bacillus typhosus has been determined. Many substances have been found with especial restraining power for the tubercle bacillus under the conditions of the test. This capacity to restrain growth in the case of the tubercle bacillus apparently bears no simple relation to true disinfectant action. Opi...

We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in...